Transcriptomics

Dataset Information

2

Iron regulation in P. syringae DC3000


ABSTRACT: DC3000 cultures were grown under highly controlled conditions and after the addition of iron citrate or sodium citrate to the media. In the cultures supplemented with iron, we found that cell-associated iron increased rapidly while culture densities were not significantly different over 4 hours when compared to cultures with sodium citrate added. Microarray analysis of samples taken from before and after the addition of either sodium citrate or iron citrate identified 386 differentially regulated genes with high statistical confidence. Differentially regulated genes were clustered based on expression patterns observed between comparison of samples taken at different time points and with different supplements. This analysis grouped genes associated with the same regulatory motifs and/or had similar putative or known function. Keywords: iron response, environmental signal, time course 5 biological replicates, 3 timepoint: 0h, 0.5h, 4h after addition of iron citrate or sodium citrate to increase final concentration by 50uM

ORGANISM(S): Pseudomonas syringae pv. tomato str. DC3000  

SUBMITTER: Michael Rutzke   Samuel W Cartinhour  Chris R Myers  Philip A Bronstein  David J Schneider  Philip Alan Bronstein  Melanie J Filialtrault 

PROVIDER: E-GEOD-13500 | ArrayExpress | 2008-11-07

SECONDARY ACCESSION(S): GSE13500PRJNA110097

REPOSITORIES: GEO, ArrayExpress

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Publications

Global transcriptional responses of Pseudomonas syringae DC3000 to changes in iron bioavailability in vitro.

Bronstein Philip A PA   Filiatrault Melanie J MJ   Myers Christopher R CR   Rutzke Michael M   Schneider David J DJ   Cartinhour Samuel W SW  

BMC microbiology 20081202


BACKGROUND: Pseudomonas syringae pv tomato DC3000 (DC3000) is a Gram-negative model plant pathogen that is found in a wide variety of environments. To survive in these diverse conditions it must sense and respond to various environmental cues. One micronutrient required for most forms of life is iron. Bioavailable iron has been shown to be an important global regulator for many bacteria where it not only regulates a wide variety of genes involved in general cell physiology but also virulence det  ...[more]

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