Transcriptomics

Dataset Information

2

H. ducreyi 35000HP grown in the presence or absence of FCS


ABSTRACT: Haemophilus ducreyi 35000HP was subjected to different in vitro growth conditions that resemble the in vivo host environment (presence of serum), in order to ascertain the overall changes in gene expression. This information might identify genes important for colonization and/or survival inside the host. H. ducreyi 35000HP was grown for 8 hours in Columbia broth in the presence or absence of FCS. After 8 hours total RNA was isolated and processed for DNA microarray analysis. This study includes four biological replicates (paired samples of bacteria grown in the presence/absence of FCS), one of which (Rep1) was selected for dye swap.

ORGANISM(S): [Haemophilus] ducreyi 35000HP  

SUBMITTER: Eric J Hansen   Maria Labandeira-Rey 

PROVIDER: E-GEOD-13851 | ArrayExpress | 2010-05-18

SECONDARY ACCESSION(S): GSE13851PRJNA110533

REPOSITORIES: GEO, ArrayExpress

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Publications

Regulation of expression of the Haemophilus ducreyi LspB and LspA2 proteins by CpxR.

Labandeira-Rey Maria M   Mock Jason R JR   Hansen Eric J EJ  

Infection and immunity 20090518 8


The LspA1, LspA2, and LspB proteins of Haemophilus ducreyi comprise a two-partner secretion system that has been shown to be necessary for H. ducreyi to inhibit phagocytosis by immune cells in vitro. Inactivation of lspA1 resulted in increased levels of LspA2, suggesting that these two proteins are differentially controlled (C. J. Ward et al., Infect. Immun. 71:2478-2486, 2003). Expression of LspA2 but not LspA1 was shown to be both growth phase dependent and affected by the presence of fetal ca  ...[more]

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