Transcriptomics

Dataset Information

6

A microRNA microarray analysis of C.elegans Parkinsons disease models


ABSTRACT: MicroRNAs (miRNAs) play an important role in human brain development and maintenance. To search for miRNAs potentially involved with molecular mechanisms in the pathogenesis of Parkinsons disease (PD), we utilized miRNA microarrays to identify expression changes of 115 annotated Caenorhabditis elegans (C.elegans) miRNAs in human α-synuclein A53T transgenic, dopamine deficient catecholamine transporter gene cat-1 mutant and parkin gene pdr-1 mutant C.elegans strains. Twelve miRNAs were found regulated differentially in α-synuclein transgenic C. elegans, five in cat-1 mutants and three in pdr-1 mutants. The family of miR64/65 appeared co-under-expressed in α-synuclein and cat-1 strains and members of let-7 family co-under-expressed (except miR-241 over-expressed) in a-synuclein and pdr-1 strains. Class H serpentine receptor (srh) family of G-protein-coupled receptor genes were computationally identified to be highly over-represented target candidates for regulated miRNAs.These results indicate that miRNAs are misregulated in C. elegans PD models and suggest a role for these molecules in the disease pathogenesis. Two color NCode microRNA microrarrays were used (Invitrogen) to measure miRNA expression changes in human alpha-synuclein trangenic, cat-1 mutant, and pdr-1 mutant C.elegans strains. The experiment contains four independent biological replicates (worm populations)/strain. Strain N2 was used as reference channel, also prepared from four independently isolated small RNA samples.

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: Richard Nass   Martina Rudgalvyte  Kristiina Louhiranta  Suvi Asikainen  Garry Wong  Merja Lakso  Suvi Tuulia Asikainen 

PROVIDER: E-GEOD-14899 | ArrayExpress | 2010-06-20

SECONDARY ACCESSION(S): GSE14899PRJNA111999

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Global microRNA expression profiling of Caenorhabditis elegans Parkinson's disease models.

Asikainen Suvi S   Rudgalvyte Martina M   Heikkinen Liisa L   Louhiranta Kristiina K   Lakso Merja M   Wong Garry G   Nass Richard R  

Journal of molecular neuroscience : MN 20100121 1


MicroRNAs (miRNAs) play an important role in human brain development and maintenance. To search for miRNAs that may be involved in the pathogenesis of Parkinsons disease (PD), we utilized miRNA microarrays to identify potential gene expression changes in 115 annotated miRNAs in PD-associated Caenorhabditis elegans models that either overexpress human A53T alpha-synuclein or have mutations within the vesicular catecholamine transporter (cat-1) or parkin (pdr-1) ortholog. Here, we show that 12 spe  ...[more]

Similar Datasets

2009-11-01 | GSE14899 | GEO
2010-07-16 | E-CBIL-46 | ArrayExpress
2010-06-11 | E-GEOD-6586 | ArrayExpress
2013-10-28 | E-GEOD-51781 | ArrayExpress
| GSE9807 | GEO
2010-08-17 | E-GEOD-16587 | ArrayExpress
| GSE49036 | GEO
2010-06-11 | E-GEOD-4723 | ArrayExpress
2016-03-15 | E-MTAB-3986 | ArrayExpress
2010-07-21 | E-GEOD-19534 | ArrayExpress