Transcriptomics

Dataset Information

2

MicroRNA expression in bone marrow cells from Gfi1 deficient mice


ABSTRACT: Growth factor independent-1 (Gfi1) is a zinc finger transcription factor with a SNAG amino-terminal repressor domain and is critically required for normal myelopoesis. Gfi1 is normally expressed in HSCs and induced during differentiation from CMPs to GMPs. Gfi1 loss-of-function mutation in mice and humans induces an arrest during myeloid differentiation and an absolute block to the formation mature neutrophils. This comparative microarray study was initiated to identify microRNAs which are potentially regulated by Gfi1 during granulopoiesis. The Gfi1 null allele from Orkin (deletion of exons 2 and 3) was backcrossed to B6 or Balb/c mice for at least 7 generations. RNA was isolated from low density bone marrow samples from Gfi1+/+ and Gfi1-/- B6 littermates, as well as Gfi1+/+ and Gfi1-/- Balb/c littermates.

ORGANISM(S): Mus musculus  

SUBMITTER: C S Velu   A M Baktula  Terry Camerlengo  H L Grimes 

PROVIDER: E-GEOD-15077 | ArrayExpress | 2009-03-10

SECONDARY ACCESSION(S): GSE15077PRJNA114773

REPOSITORIES: GEO, ArrayExpress

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Publications

Gfi1 regulates miR-21 and miR-196b to control myelopoiesis.

Velu Chinavenmeni S CS   Baktula Avinash M AM   Grimes H Leighton HL  

Blood 20090310 19


The zinc finger protein growth factor independent-1 (Gfi1) is a transcriptional repressor that is critically required for normal granulocytic differentiation. GFI1 loss-of-function mutations are found in some patients with severe congenital neutropenia (SCN). The SCN-associated GFI1-mutant proteins act as dominant negatives to block granulopoiesis through selective deregulation of a subset of GFI1 target genes. Here we show that Gfi1 is a master regulator of microRNAs, and that deregulated expre  ...[more]

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