Transcriptomics

Dataset Information

2

Induction of Pluripotency in Adult Unipotent Germline Stem Cells


ABSTRACT: Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germline-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time. Nine samples were analyzed. GSC1: Mouse Germ Stem Cells, line 1 (1 replicate), GSC2: Mouse Germ Stem Cells, line 2 (1 replicate), GSC3: Mouse Germ Stem Cells, line 3 (1 replicate), GSC4: Mouse Germ Stem Cells, line 4 (1 replicate) gPS1: Mouse clonal germ Pluripotent Stem cells, line 1, GFP-sorted (1 replicate), gPS2: Mouse clonal germ Pluripotent Stem cells, line 2, GFP-sorted (1 replicate), gPS3: Mouse clonal germ Pluripotent Stem cells, line 3, GFP-sorted (1 replicate) ESC: Mouse Embryonic Stem Cells (duplicate)

ORGANISM(S): Mus musculus  

SUBMITTER: Tamara Glaser   Philipp Sasse   Kinarm Ko  Marcos J Arauzo-Bravo  Martin Zenke  Guangming Wu  Oliver Brüstle  Bernd K Fleischmann  Boris Greber  Natalia Tapia  David Ruau  Dong-Wook Han  Kirsten Hausdörfer  Jeong-Beom Kim  Vittorio Sebastiano  Martin Stehling  Marcos J Araúzo-Bravo  Hans R Schöler 

PROVIDER: E-GEOD-16178 | ArrayExpress | 2010-04-09

SECONDARY ACCESSION(S): GSE16178PRJNA115067

REPOSITORIES: GEO, ArrayExpress

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Publications


Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germline-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of  ...[more]

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