Transcriptomics

Dataset Information

3

Gene expression data from endothelial cells and leukocytes enriched from transplanted rat hearts


ABSTRACT: Transcriptome analyses of organ transplants have until now usually focused on whole tissue samples containing activation profiles from different cell populations. Here, we enriched endothelial cells from rat cardiac allografts and isografts, establishing their activation profile at baseline and on day 2, 3 and 4 after transplantation. Modulated transcripts were assigned to three categories based on their regulation profile in allografts and isografts. Categories A and B contained the majority of transcripts and showed similar regulation in both graft types, appearing to represent responses to surgical trauma. By contrast, category C contained transcripts that were partly allograft-specific and to a large extent associated with interferon--responsiveness. Several transcripts were verified by immunohistochemical analysis of graft lesions, among them the matricellular protein periostin which was one of the most highly upregulated transcripts but has not been associated with transplantation previously. In conclusion, the majority of the differentially expressed genes in graft endothelial cells are affected by the transplantation procedure whereas relatively few are associated with allograft rejection. Endothelial cells were enriched from control/non-transplanted hearts and from allografts (DA to Lewis) and isografts (Lewis to Lewis) harvested at day 2, 3 and 4 after transplantation. Three biological replicates from each time point and graft type were analyzed (a total of 24 arrays). Leukocytes were enriched from allografts at day 2 (n=1), day 3 (n=2) and day 4 (n=1) after transplantation.

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Junbai Wang  Pål-Dag Line  Guttorm Haraldsen  Monika Kasprzycka  Helge Scott  Bjarte Fosby  Hilde Bjærke  Bjørg Mikalsen  Clara Hammarström  Marlene Lundström 

PROVIDER: E-GEOD-16695 | ArrayExpress | 2010-07-29

SECONDARY ACCESSION(S): GSE16695PRJNA117379

REPOSITORIES: GEO, ArrayExpress

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