Transcriptomics

Dataset Information

0

Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush


ABSTRACT: Retinal ganglion cell (RGC) death is the final consequence of many blinding diseases, where there is considerable variation in the time course and severity of RGC loss. Indeed, this process appears to be influenced by a wide variety of genetic and environmental factors. In this study we explored the genetic basis for differences in ganglion cell death in two inbred strains of mice. We found that RGCs are more susceptible to death following optic nerve crush in C57BL/6J mice (54% survival) than in DBA2/J mice (62% survival). Using the Illumina Mouse-6 microarray, we identified 1,580 genes with significant change in expression following optic nerve crush in these two strains of mice. Our analysis of the changes occurring after optic nerve crush demonstrated that the greatest amount of change (44% of the variance) was due to the injury itself. This included changes associated with ganglion cell death, reactive gliosis, and abortive regeneration. The second pattern of gene changes (23% of the variance) was primarily related to differences in gene expressions observed between the C57BL/6J and DBA/2J mouse strains. The remaining changes in gene expression represent interactions between the effects of optic nerve crush and the genetic background of the mouse. We extracted one genetic network from this dataset that appears to be related to tissue remodeling. One of the most intriguing sets of changes included members of the crystallin family of genes, which may represent a signature of pathways modulating the susceptibility of cells to death. Differential responses to optic nerve crush between two widely used strains of mice were used to define molecular networks associated with ganglion cell death and reactive gliosis. These results form the basis for our continuing interest in the modifiers of retinal injury. 18 Samples: 9 per strain (C57BL/6J & DBA/2J); 3 conditions per strain

ORGANISM(S): Mus musculus  

SUBMITTER: Justin P Templeton  Eldon E Geisert   Felix Vazquez-Chona   Mohamed Nassr   William E Orr   Robert W Williams   William Edwin Orr   Natalie E Freeman-Anderson    

PROVIDER: E-GEOD-17117 | ArrayExpress | 2010-08-19

SECONDARY ACCESSION(S): GSE17117PRJNA119831

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush.

Templeton Justin P JP   Nassr Mohamed M   Vazquez-Chona Felix F   Freeman-Anderson Natalie E NE   Orr William E WE   Williams Robert W RW   Geisert Eldon E EE  

BMC neuroscience 20090730


Retinal ganglion cell (RGC) death is the final consequence of many blinding diseases, where there is considerable variation in the time course and severity of RGC loss. Indeed, this process appears to be influenced by a wide variety of genetic and environmental factors. In this study we explored the genetic basis for differences in ganglion cell death in two inbred strains of mice.We found that RGCs are more susceptible to death following optic nerve crush in C57BL/6J mice (54% survival) than in  ...[more]

Similar Datasets

2013-07-15 | E-GEOD-40857 | ArrayExpress
2010-02-21 | E-GEOD-19298 | ArrayExpress
2016-08-24 | PXD001581 | Pride
2014-02-21 | E-GEOD-55228 | ArrayExpress
2009-07-16 | GSE17117 | GEO
2008-10-20 | E-GEOD-9918 | ArrayExpress
2011-10-27 | E-GEOD-32309 | ArrayExpress
2008-06-14 | E-MEXP-1387 | ArrayExpress
2015-07-22 | E-GEOD-67130 | ArrayExpress
2010-02-01 | GSE19298 | GEO