Transcriptomics

Dataset Information

6

Rapid Identification of Bacterial Pathogens using a PCR- and Microarray-Based Assay


ABSTRACT: A method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples. The processed data are composed of ORGANISM NAME or NEGATIVE answer depending if the pathogen is detected in the sample or not. See supplementary file linked below.

SUBMITTER: Minna Maki  Anna-Kaarina Jarvinen 

PROVIDER: E-GEOD-17221 | ArrayExpress | 2010-06-24

SECONDARY ACCESSION(S): GSE17221PRJNA119185

REPOSITORIES: GEO, ArrayExpress

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Publications

Rapid identification of bacterial pathogens using a PCR- and microarray-based assay.

Järvinen Anna-Kaarina AK   Laakso Sanna S   Piiparinen Pasi P   Aittakorpi Anne A   Lindfors Merja M   Huopaniemi Laura L   Piiparinen Heli H   Mäki Minna M  

BMC microbiology 20090810


BACKGROUND: During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The pri  ...[more]

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