Transcriptomics

Dataset Information

4

Expresson from human THP-1 stimulated with IFN-g and/or infected with TB


ABSTRACT: Investigation of interacting effects of TB and IFN-g on gene expression regulation within human macrophage-like THP-1 cells To investigate the effect TB infection has on THP-1 macrophage’s response to IFN-g post-infection, in multiple experiments, THP-1 cells were exposed to each of the following four conditions: not treated, IFN-γ treated, M. tuberculosis infected and M. tuberculosis infected then IFN-γ treated. Total cellular RNA was then extracted. Recovered RNAs were pre-qualified for reproducibility by comparing qRT-PCR results of IFIT-2 and IP10 between replicate experiments. Four most reproducible replicate experiments were then selected for genome-wide mRNA abundance assays using Affymetrix microarray-based technology.

ORGANISM(S): Homo sapiens  

SUBMITTER: Richard Pine  

PROVIDER: E-GEOD-17477 | ArrayExpress | 2010-03-09

SECONDARY ACCESSION(S): GSE17477PRJNA118703

REPOSITORIES: GEO, ArrayExpress

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