Transcriptomics

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Effects of exercise on miRNA expression level in human neutrophils


ABSTRACT: Exercise leads to a rapid change in the profile of gene expression in circulating neutrophils. We hypothesized that miRNA expression in circulating neutrophils would also be affected by brief exercise. Eleven healthy men (19-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 76% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. Neutrophils were isolated using OptiPrep Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol®. For this study we used Agilent Human miRNA microarrays V2 (total of 22 chips).

ORGANISM(S): Homo sapiens  

SUBMITTER: Shlomit Radom-Aizik   Dan M Cooper  Frank Zaldivar 

PROVIDER: E-GEOD-18999 | ArrayExpress | 2010-03-31

SECONDARY ACCESSION(S): GSE18999PRJNA120429

REPOSITORIES: GEO, ArrayExpress

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Publications

Evidence for microRNA involvement in exercise-associated neutrophil gene expression changes.

Radom-Aizik Shlomit S   Zaldivar Frank F   Oliver Stacy S   Galassetti Pietro P   Cooper Dan M DM  

Journal of applied physiology (Bethesda, Md. : 1985) 20100128 1


Exercise leads to a rapid change in the profile of gene expression in circulating neutrophils. MicroRNAs (miRNAs) have been discovered to play important roles in immune function and often act to attenuate or silence gene translation. We hypothesized that miRNA expression in circulating neutrophils would be affected by brief exercise. Eleven healthy men (19-30 yr old) performed 10, 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to approximately  ...[more]

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