Transcriptomics

Dataset Information

63

Development of molecular genetic analysis tools for the domestic ferret (Mustela putorius furo)


ABSTRACT: The domestic ferret (Mustela putorius furo) has been used as animal model for decades, largely because its susceptibility to infection with a large number of pathogens such as influenza virus, SARS Corona virus and Canine distemper virus. Despite its importance for biomedical research, little is known about the genome of the M. Furo. The number of reagents for molecular and immunological analysis is thus restricted. To circumvent this, we present here a parallel sequencing effort to produce an extensive EST dataset derived from a normalized ferret cDNA library made from mRNA from ferret blood, liver, lung, spleen and brain. We produced more than 500000 sequence reads that were assembled into over 15000 partial ferret transcripts. These ESTs were combined with the available ferret sequences in the GenBank to develop a ferret specific microarray platform. Using this array, we detected tissue specific expression patterns which were confirmed by quantitative real time PCR assays and comparison to orthologous transcription profiles of mouse and human. We also present a set of 41 ferret transcript with even transcription profile across the tested tissues, indicating their usefulness as housekeeping genes. This study paves way for development of additional reagents for analysis of the ferret model. Three biological replicates of blood, lung, spleen, liver and brain was hybridized to the ferret specific microarray.

ORGANISM(S): Mustela putorius furo  

SUBMITTER: Willy Valdivia Granda   Ronald Tapp  Francis Larson  Colleen B. Jonsson  Alexis McBrayer  Suxia Yao  Nicholas Powers  Carl E Bruder  Jeremy Camp 

PROVIDER: E-GEOD-19398 | ArrayExpress | 2010-09-13

SECONDARY ACCESSION(S): GSE19398PRJNA121761

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptome sequencing and development of an expression microarray platform for the domestic ferret.

Bruder Carl E CE   Yao Suxia S   Larson Francis F   Camp Jeremy V JV   Tapp Ronald R   McBrayer Alexis A   Powers Nicholas N   Granda Willy Valdivia WV   Jonsson Colleen B CB  

BMC genomics 20100419


The ferret (Mustela putorius furo) represents an attractive animal model for the study of respiratory diseases, including influenza. Despite its importance for biomedical research, the number of reagents for molecular and immunological analysis is restricted. We present here a parallel sequencing effort to produce an extensive EST (expressed sequence tags) dataset derived from a normalized ferret cDNA library made from mRNA from ferret blood, liver, lung, spleen and brain.We produced more than 5  ...[more]

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