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Phenotypic plasticity in the pea aphid Acyrthosiphon pisum: miRNA sequencing

ABSTRACT: Bioinformatic prediction, deep sequencing of microRNA and expression analysis during phenotypic plasticity in the pea aphid acyrthosiphon pisum We developed high throughput Solexa sequencing and bioinformatic analyses of the genome of the pea aphid Acyrthosiphon pisum in order to identify the first miRNAs from a hemipteran insect. By combining these methods we identified 155 miRNAs including 56 conserved and 99 new miRNAs. Moreover, we investigated the regulation of these miRNAs in different alternative morphs of the pea aphid by analysing the expression of miRNAs across the switch of reproduction mode. deep sequencing of small RNAs from parthenogenetic Acyrthosiphon pisum

ORGANISM(S): Acyrthosiphon pisum  

SUBMITTER: Owain Edwards   Shuji Shigenobu  Stephanie Jaubert  Tom Walsh 

PROVIDER: E-GEOD-20107 | ArrayExpress | 2010-03-01



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Bioinformatic prediction, deep sequencing of microRNAs and expression analysis during phenotypic plasticity in the pea aphid, Acyrthosiphon pisum.

Legeai Fabrice F   Rizk Guillaume G   Walsh Thomas T   Edwards Owain O   Gordon Karl K   Lavenier Dominique D   Leterme Nathalie N   Méreau Agnès A   Nicolas Jacques J   Tagu Denis D   Jaubert-Possamai Stéphanie S  

BMC genomics 20100505

BACKGROUND: Post-transcriptional regulation in eukaryotes can be operated through microRNA (miRNAs) mediated gene silencing. MiRNAs are small (18-25 nucleotides) non-coding RNAs that play crucial role in regulation of gene expression in eukaryotes. In insects, miRNAs have been shown to be involved in multiple mechanisms such as embryonic development, tissue differentiation, metamorphosis or circadian rhythm. Insect miRNAs have been identified in different species belonging to five orders: Coleop  ...[more]

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