Quadruple rrm experience - Transcriptomic study of a quadruple rrm mutant which belong to a new family of AGO hook proteins
ABSTRACT: rs10-01_rrm - quadruple rrm experience - What is the role of the RRM protein family in plants? Plants were grown on soil in controlled environment under LD (16 h light/8 h dark) and the rosette leaves (8-leaf stage seedlings) were collected for RNA preparation. Keywords: normal vs transgenic comparison 2 dye-swap - CATMA arrays
Project description:gnp06-03_microtrac Endogenous hairpins-ir71 targets What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants? Lines IR71 KO n°3 and Col-0 were grown on soil until bolting, Flower buds were then harvested and RNA extracted with Qiagen RNeasy Plant mini kit. RNA was stored at -20°C. 3 dye-swap - gene knock out
Project description:What is the physiological function of cytochrome b561 in Arabidopsis thaliana - We want to check which possible changes occur in gene expression by knocking out the AtCytb1 gene. au08-01_cytb561 - cytb1-1 Keywords: gene knock out 4 dye-swap - CATMA arrays
Project description:ra09-02_tctp - rnai - Identification of effector genes that act downstream of AtTCTP, using knockout ( tctp-2), RNAi expressing line (35S::RNAi-AtTCTP) and over expressing line (35S::AtTCTP) - transcriptome analysis in leaf of 35S::RNAi-AtTCTP versus wild type Keywords: normal vs rnai mutant comparison 2 dye-swap - CATMA arrays
Project description:adt10-01_wip2 - wip overexpressing plants - Identification of genes induced or repressed by WIP genes - The objective is to identify genes responsible for the phenotypes observed in WIP overexpressing lines. WIP transcription factors are involved in reproductive and vegetative development by acting on the remodeling of various tissues. The WIP related processes have a major impact, in particular on reproduction (sex determination, fertilization, development of the seed). The way of action of these transcription factors is probably conserved, their effect depending only on their specificity of expression. Two WIP genes, NTT and TT1, was overexpressed in Arabidopsis thaliana. The objective is to compare the common deregulated genes (and in particular repressed) in the transcriptome of these two overexpressor lines, to highlight their mode of action and the way they act on the tissue in which they are expressed. Keywords: gene knock in (transgenic),normal vs transgenic comparaison 6 dye-swap - CATMA arrays
Project description:ra08-03_kim - laccases - Are laccases involved in lignification - all plants were harveted at the same developmental stage in the same growth chamber between 10h30 and 11h. Keywords: wt vs mutant comparison 8 dye-swap - CATMA arrays
Project description:ra09-03_sgs - mutants versus wt - Identification of new genes regulated by SGS1 and SGS6/UBP5 (two genes involved in PTGS) by analysis of the transcriptomes of sgs1 and sgs6 mutants compared to wild-type plants in the shoots of seedlings. The comparison between the transcriptomes of sgs3, chr11, chr17 simple mutants impaired differently in PTGS and development (juvenile-to-adult transition and bolting), and the transcriptomes of the sgs3 chr11, sgs3 chr17 double mutants showed additive effects on development (juvenile-to-adult transition and bolting), which allows a better understanding of the genetic link existing between SGS3 and its potential partners, CHR11 (which interacts with SGS3 in a two-hybrid assay and BiFC) and CHR17, 2 homologous proteins of the SWI/SNF2 family. Transcriptome comparison between mutants and wild-type plants L1 in the shoots of seedlings. 14 dye-swaps - gene knock-in (transgenic).
Project description:rs08-03_glutathion - glutathion - How does glutathione content or reduction state affect H2O2-induced changes in the transcriptome? - Three single Arabidopsis mutants were used: cat2, knockout for catalase2 and so enriched in H2O2; cad2, defective in glutathione content; cytGR, knockout for cytosolic glutathione reductase. Cat2 was crossed with cad2 and cyt GR, and col0, 3 single mutants, and 2 double mutants were sampled in controlled growth conditions either in 8h or 16h photoperiod. Keywords: gene knock out 20 dye-swap pairs - CATMA arrays: 40 arrays
Project description:rs09-10_fwa - wt vs mutants 09. Impact of loss of DNA methylation and small interference RNAs (siRNAs) in gene expression. Determination of the genes misregulated upon ectopic expression of the FWA imprinted gene. Mutants impaired for the DNA methylation maintenance pathway and RNA interference machinery were compared to mutants simultaneously impaired for both. Impact of FWA and SDC ectopic expression. 15 dye-swaps. fwa-d, gain of function epimutation, gene knock-in (transgenic), gene knockout.
Project description:adt09-02_fireman - fireman vs wt erwinia infection - A meta-analysis ot the CATMA transcriptomic data has shown that an orphan gene of Arabidopsis: AT5E39460, recently remaned AT5G 44585, is highly responsive to biotic stresses. An homozygous mutant of this gene, named fireman has been produced and displays less cell death symptoms in comparison to the wild type in response to Erwinia amylovora infection. The purpose of this transcriptomic analysis is to understand which pathways are altered in this mutant versus the wild-type in response to this phytopathogenic bacteria. - 3 inoculated leaves of 5 weeks old plants, per plant, have been harvested 24h after inoculation with H20 or E. amylovora, strain 1430. Keywords: normal vs disease comparison, normal vs transgenic comparaison 8 dye-swap - CATMA arrays