Transcriptomics

Dataset Information

2

Different propensity in hematopoietic differentiation potential among distinct human pluripotent stem cell lines


ABSTRACT: Lineage-specific differentiation potential varies among different human pluripotent stem cell (hPSC) lines. A stem cell bank may advice researchers on which hPSCs exhibit the highest differentiation potential for a certain lineage. In this study, we aimed at characterizing the hematopoietic differentiation potential from 14 hESC/iPSC lines through the embryoid body (hEB) differentiation system. We carried out gene expression profiling on six different hESC lines grouped according to their ability to differentiate towards hematopoietic lineage: SHEF1, AND1 and H1 were considered as good (high CD45 expression and high CFU potential), whereas H9, HS181 and VAL3 were selected as poor-blood differentiating lines (low CD45 expression and low CFU potential). Human ESC samples were collected during the exponential cell growth phase and stabilized in RNA later. 500 ng of each total RNA sample was labelled with Cy3 using the Quick-Amp Labelling kit and hybridized with the Gene Expression Hybridization kit to a Whole Human Genome Oligo Microarray (Agilent Technologies) following the Manufacturer’s instructions. Each cell line was analyzed as independent duplicates. H9 hESC line was used as the baseline.

ORGANISM(S): Homo sapiens  

SUBMITTER: Verónica Ramos-Mejía   Clara Bueno  Jose L Cortés  Gustavo J Melen  Pablo Menéndez  Pedro J Real  Laura Sánchez  Iván Gutiérrez-Aranda  Gertrudis Ligero 

PROVIDER: E-GEOD-23091 | ArrayExpress | 2010-07-24

SECONDARY ACCESSION(S): GSE23091PRJNA131875

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Nodal/Activin signaling predicts human pluripotent stem cell lines prone to differentiate toward the hematopoietic lineage.

Ramos-Mejia Veronica V   Melen Gustavo J GJ   Sanchez Laura L   Gutierrez-Aranda Ivan I   Ligero Gertrudis G   Cortes Jose L JL   Real Pedro J PJ   Bueno Clara C   Menendez Pablo P  

Molecular therapy : the journal of the American Society of Gene Therapy 20100824 12


Lineage-specific differentiation potential varies among different human pluripotent stem cell (hPSC) lines, becoming therefore highly desirable to prospectively know which hPSC lines exhibit the highest differentiation potential for a certain lineage. We have compared the hematopoietic potential of 14 human embryonic stem cell (hESC)/induced pluripotent stem cell (iPSC) lines. The emergence of hemogenic progenitors, primitive and mature blood cells, and colony-forming unit (CFU) potential was an  ...[more]

Similar Datasets

| GSE23091 | GEO
2014-09-03 | E-GEOD-61017 | ArrayExpress
2010-12-03 | E-GEOD-20045 | ArrayExpress
2013-06-04 | E-GEOD-40103 | ArrayExpress
2013-08-31 | E-GEOD-48257 | ArrayExpress
| PRJNA347537 | ENA
2011-06-09 | E-GEOD-29869 | ArrayExpress
| GSE48257 | GEO
| GSE20013 | GEO
2015-02-01 | E-GEOD-62501 | ArrayExpress