Transcriptomics

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Transcription profiling by array of Arabidopsis mutant for abi4 and/or vtc2


ABSTRACT: The role of abscisic acid (ABA) signalling in the ascorbic acid (AA)-dependent control of plant growth and defence was determined using the vtc1 and vtc2 mutants, which have impaired ascorbic acid synthesis, and in the abi4 mutant that is impaired in ABA-signalling. ABA levels were increase in the mutants relative to the wild type (Col0). Like vtc1 the vtc2 mutants have a slow growth relative to Col0. However, the wild type phenotype is restored in the abi4vtc2 double mutant. Similarly, the sugar sensing phenotype of in the abi4 is reversed in the abi4vtc2 double mutant. The vtc1 and vtc2 leaf transcriptomes show up to 70 % homology with abi4. Of the transcripts that are altered in the mutants a relative to Col0, only a small number are reversed in the abi4vtc2 double mutants relative to either abi4 or vtc2. We conclude that AA controls growth via an ABA and abi4-dependent signalling pathway. The vtc and abi4 mutants have enhanced glutathione levels and common redox signalling pathways leading to similar gene expression patterns. Rosettes of 42 days old plants were harvested and used to exctract RNA

REANALYSED by: E-GEOD-23329

OTHER RELATED OMICS DATASETS IN: E-GEOD-23330

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Christine H Foyer   Pavel Kerchev 

PROVIDER: E-GEOD-23329 | ArrayExpress | 2011-08-25

SECONDARY ACCESSION(S): GSE23329PRJNA133295

REPOSITORIES: GEO, ArrayExpress

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Publications

The transcription factor ABI4 Is required for the ascorbic acid-dependent regulation of growth and regulation of jasmonate-dependent defense signaling pathways in Arabidopsis.

Kerchev Pavel I PI   Pellny Till K TK   Vivancos Pedro Diaz PD   Kiddle Guy G   Hedden Peter P   Driscoll Simon S   Vanacker Hélène H   Verrier Paul P   Hancock Robert D RD   Foyer Christine H CH  

The Plant cell 20110916 9


Cellular redox homeostasis is a hub for signal integration. Interactions between redox metabolism and the ABSCISIC ACID-INSENSITIVE-4 (ABI4) transcription factor were characterized in the Arabidopsis thaliana vitamin c defective1 (vtc1) and vtc2 mutants, which are defective in ascorbic acid synthesis and show a slow growth phenotype together with enhanced abscisic acid (ABA) levels relative to the wild type (Columbia-0). The 75% decrease in the leaf ascorbate pool in the vtc2 mutants was not suf  ...[more]

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