Transcriptomics

Dataset Information

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PCPTC screening


ABSTRACT: Primary cultures of patient tumor cells (PCPTC) were used in a cell-based cytotoxicity screen. Microarray-based mRNA profiling was used to identify the mechanism-of-action for the small molecule VLX 50. MCF7 cells were treated with the PCPTC screening hit VLX 50 or DMSO control for 6h prior to RNA isolation. One sample per treatment. Data were analyzed using both MAS5.0 and RMA.

ORGANISM(S): Homo sapiens  

SUBMITTER: Mårten Fryknäs   Marten Fryknas 

PROVIDER: E-GEOD-24027 | ArrayExpress | 2011-09-08

SECONDARY ACCESSION(S): GSE24027PRJNA130273

REPOSITORIES: GEO, ArrayExpress

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Publications

Phenotype-based drug screening in primary ovarian carcinoma cultures identifies intracellular iron depletion as a promising strategy for cancer treatment.

Gullbo Joachim J   Fryknäs Mårten M   Rickardson Linda L   Darcy Padraig P   Hägg Maria M   Wickström Malin M   Hassan Sadia S   Westman Gunnar G   Brnjic Slavica S   Nygren Peter P   Linder Stig S   Larsson Rolf R  

Biochemical pharmacology 20110422 2


Primary cultures of patient tumor cells (PCPTC) have been used for prediction of diagnosis-specific activity and individual patient response to anticancer drugs, but have not been utilized as a model for identification of novel drugs in high throughput screening. In the present study, ovarian carcinoma cells from three patients were tested in response to a library of 3000 chemically diverse compounds. Eight hits were retrieved after counter screening using normal epithelial cells, and one of the  ...[more]

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