Transcriptomics

Dataset Information

7

Genomic Collaboration of Estrogen Receptor-α and ERK2 in Regulating Gene and Proliferation Programs


ABSTRACT: The nuclear hormone receptor, estrogen receptor-alpha (ERα), and MAP kinases both play key roles in hormone-dependent cancers, yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ERα activates and interacts with ERK2, a downstream effector in the MAPK pathway, resulting in ERK2 and ERα colocalization at chromatin binding sites across the genome of breast cancer cells. KEYWORDS: siRNA knock-down, ligand treatment MCF-7 human breast adenocarcinoma cells were tranfected with control, ERK1 and ERK2 siRNA for 60 hours and treated with 0.1% EtOH (Vehicle) or 10 nM E2 for 4 hours or 24 hours, and cDNA microarray analyses were carried out using Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array.

REANALYSED by: E-GEOD-24592

ORGANISM(S): Homo sapiens  

SUBMITTER: Zeynep Madak Erdogan   Zeynep M Erdogan  Benita Katzenellenbogen 

PROVIDER: E-GEOD-24592 | ArrayExpress | 2011-01-10

SECONDARY ACCESSION(S): GSE24592PRJNA132379

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Genomic collaboration of estrogen receptor alpha and extracellular signal-regulated kinase 2 in regulating gene and proliferation programs.

Madak-Erdogan Zeynep Z   Lupien Mathieu M   Stossi Fabio F   Brown Myles M   Katzenellenbogen Benita S BS  

Molecular and cellular biology 20101018 1


The nuclear hormone receptor, estrogen receptor α (ERα), and mitogen-activated protein kinases (MAPKs) play key roles in hormone-dependent cancers, and yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ERα activates and interacts with extracellular signal-regulated kinase 2 (ERK2), a downstream effector in the MAPK pathway, resulting in ERK2 and ERα colocalization at chromatin binding sites across the  ...[more]

Similar Datasets

2015-08-12 | E-GEOD-24592 | ExpressionAtlas
2011-01-10 | GSE24592 | GEO
2014-06-03 | E-GEOD-53394 | ArrayExpress
2013-07-05 | E-GEOD-42347 | ArrayExpress
2014-08-07 | E-GEOD-55204 | ArrayExpress
2013-04-04 | E-GEOD-42376 | ArrayExpress
2016-10-25 | E-GEOD-78284 | ArrayExpress
2019-02-09 | PXD010314 | Pride
2013-09-01 | E-GEOD-47803 | ArrayExpress
2010-04-09 | E-GEOD-14847 | ArrayExpress