Transcriptomics

Dataset Information

302

X chromosome dosage compensation via enhanced transcriptional elongation in Drosophila males (Untreated)


ABSTRACT: MSL (Male-specific lethal) complex increases transcription on the single X chromosome of Drosophila males in order to equalize expression of X-linked genes between males (XY) and females (XX). The increase in transcript levels correlates with MSL- dependent acetylation of histone H4 at K16 within the bodies of active genes, but identification of the transcriptional step affected has not been possible. In this study, we use global run-on sequencing (GRO-seq) to examine the specific effect of MSL complex on RNA Polymerase II (RNAP II) on a genome-wide level. Results indicate that MSL complex enhances transcription by facilitating the progression of RNAP II across the bodies of active X-linked genes. Improving transcriptional output downstream of typical gene-specific control may explain how dosage compensation can be imposed on the diverse set of genes along an entire chromosome. Global Run-On Sequencing (GRO-Seq) reads, i.e., RNA-Seq of nascent RNA transcripts, from D. Melanogaster SL2 cells. Two biological replicates were analyzed.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Peter V Kharchenko   Peter J Park  Eric P Bishop  Leighton Core  John T Lis  Mitzi I Kuroda  Erica Larschan 

PROVIDER: E-GEOD-25321 | ArrayExpress | 2010-12-22

SECONDARY ACCESSION(S): GSE25321SRP004454PRJNA134843

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

X chromosome dosage compensation via enhanced transcriptional elongation in Drosophila.

Larschan Erica E   Bishop Eric P EP   Kharchenko Peter V PV   Core Leighton J LJ   Lis John T JT   Park Peter J PJ   Kuroda Mitzi I MI  

Nature 20110301 7336


The evolution of sex chromosomes has resulted in numerous species in which females inherit two X chromosomes but males have a single X, thus requiring dosage compensation. MSL (Male-specific lethal) complex increases transcription on the single X chromosome of Drosophila males to equalize expression of X-linked genes between the sexes. The biochemical mechanisms used for dosage compensation must function over a wide dynamic range of transcription levels and differential expression patterns. It h  ...[more]

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