Transcriptomics

Dataset Information

2

Transcriptomic profiles of Japanese medaka (Oryzias latipes) in response to alkalinity stress


ABSTRACT: Alkalinity stress is considered to be one of the major stressors for fish in saline-alkali water. Thus, it is of great significance from both aquaculture and physiological viewpoint to understand the molecular genetic response of aquatic organisms to alkalinity stress. The objective of this study is to determine genome-wide gene expression profiles to better understand the physiology response of medaka (Oryzias latipes) to high carbonate alkalinity stress. In lab-based cultures, adult fish were exposed to freshwater and high carbonate alkalinity water .We designed a microarray containing 26429 oligonucleotides and describe our experimental results for measuring gene expression changes in the gill of carbonate alkalinity stress exposed fish. The fish were exposed to freshwater (FW) and high carbonate alkalinity water (AW) for 96h, each with three replicates.

ORGANISM(S): Oryzias latipes  

SUBMITTER: Zongli Yao   Qifang Lai 

PROVIDER: E-GEOD-27298 | ArrayExpress | 2014-01-24

SECONDARY ACCESSION(S): GSE27298PRJNA137353

REPOSITORIES: GEO, ArrayExpress

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Transcriptomic profiles of Japanese medaka (Oryzias latipes) in response to alkalinity stress.

Yao Z L ZL   Wang H H   Chen L L   Zhou K K   Ying C Q CQ   Lai Q F QF  

Genetics and molecular research : GMR 20120813 3


Oryzias latipes (Adrianichthyidae), known as Japanese medaka or Japanese killifish, is a small 2-4 cm long fish common in rice paddies in coastal Southeast Asia and is also a popular aquarium fish. It has been widely used as a research model because of its small size and because it is very easy to rear. Alkalinity stress is considered to be one of the major stressors on fish in saline-alkaline water. As very little is known about molecular genetic responses of aquatic organisms to alkalin  ...[more]

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