Name: Extensive Chromatin Remodeling and Establishment of Transcription Factor 'Hotspots' during Early Adipogenesis
Keywords: ChIP-seq

Dataset Information

Extensive Chromatin Remodeling and Establishment of Transcription Factor 'Hotspots' during Early Adipogenesis

ABSTRACT: Adipogenesis is tightly controlled by a complex network of transcription factors acting at different stages of differentiation. Peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT/enhancer binding protein (C/EBP) family members are key regulators of this process. We have employed DNase I hypersensitive site analysis to investigate the genome-wide changes in chromatin structure that accompany the binding of adipogenic transcription factors. These analyses revealed a dramatic and dynamic modulation of the chromatin landscape during the first hours of adipocyte differentiation that coincides with cooperative binding of multiple early transcription factors (including glucocorticoid receptor, retinoid X receptor, Stat5a, C/EBPbeta and -delta) to transcription factor 'hotspots'. Our results demonstrate that C/EBPbeta marks a large number of these transcription factor 'hotspots' prior to induction of differentiation and chromatin remodeling and is required for their establishment. Furthermore, a subset of early remodeled C/EBP binding sites persists throughout differentiation and is later occupied by PPAR gamma , indicating that early C/EBP family members, in addition to their well established role in activation of PPAR gamma transcription, may act as pioneering factors for PPAR gamma binding. DNase I hypersensitive chromatin regions and transcription factor binding sites were identified at various time points of 3T3-L1 differentiation using DHS-seq and ChIP-seq, respectively.

ORGANISM(S): Mus musculus

SUBMITTER: Susanne Mandrup Gordon L Hager Rasmus Siersbaek Anne Loft Myong-Hee Sung Ronni Nielsen Sam John Songjoon Baek

PROVIDER: E-GEOD-27826 | ArrayExpress | 2011-03-22

SECONDARY ACCESSION(S): SRP006001GSE27826PRJNA137905

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Extensive chromatin remodelling and establishment of transcription factor 'hotspots' during early adipogenesis.

Siersbæk Rasmus R Nielsen Ronni R John Sam S Sung Myong-Hee MH Baek Songjoon S Loft Anne A Hager Gordon L GL Mandrup Susanne S

The EMBO journal 20110322 8

Adipogenesis is tightly controlled by a complex network of transcription factors acting at different stages of differentiation. Peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein (C/EBP) family members are key regulators of this process. We have employed DNase I hypersensitive site analysis to investigate the genome-wide changes in chromatin structure that accompany the binding of adipogenic transcription factors. These analyses revealed a dramatic and dynami ...[more]

PMID: 21427703

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