Transcriptomics

Dataset Information

7

Genome wide analysis reveals association of a FTO gene variant with epigenetic changes of several genes in human blood


ABSTRACT: FTO gene variants show the strongest association to obesity so far, but the role of the gene in obesity remains unclear. The FTO protein is a demethylation enzyme, which suggests that the link to obesity could be mediated through epigenetic changes. Herein, we determine the genome wide DNA methylation profile in blood from 47 female preadolescents and detect changes in five methylated regions, corresponding to six genes, associated with the FTO risk allele (rs9939609). In addition, we identify 20 differentially methylated regions associated with obesity. Our findings indicate that the effect of the FTO obesity risk allele may be mediated through epigenetic changes. Further, the identified regions might prove valuable biomarkers for the diagnosis and prognosis of obesity and comorbidites, such as cardiovascular disease. Bisulphite converted DNA from 47 girls were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2. Half of the subjects are carriers of the FTO obesity risk allele (AA) and half of the normal allele (TT). In addition, both obese and normal-weigh indiviudals were included.

ORGANISM(S): Homo sapiens  

SUBMITTER: Helgi B Schiöth   George Moschonis  George P Chrousos  Josefin A Jacobsson  Robert Fredriksson  Christian Benedict  Markus S Almén  Markus Sällman Almén 

PROVIDER: E-GEOD-27860 | ArrayExpress | 2012-01-10

SECONDARY ACCESSION(S): GSE27860PRJNA137883

REPOSITORIES: GEO, ArrayExpress

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Publications

Genome wide analysis reveals association of a FTO gene variant with epigenetic changes.

Almén Markus Sällman MS   Jacobsson Josefin A JA   Moschonis George G   Benedict Christian C   Chrousos George P GP   Fredriksson Robert R   Schiöth Helgi B HB  

Genomics 20120102 3


Variants of the FTO gene show strong association with obesity, but the mechanisms behind this association remain unclear. We determined the genome wide DNA methylation profile in blood from 47 female preadolescents. We identified sites associated with the genes KARS, TERF2IP, DEXI, MSI1, STON1 and BCAS3 that had a significant differential methylation level in the carriers of the FTO risk allele (rs9939609). In addition, we identified 20 differentially methylated sites associated with obesity. Ou  ...[more]

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