Transcriptomics

Dataset Information

0

Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells


ABSTRACT: HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. They also behave as oncogenes in some malignancies. In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. Using immunocytochemistry, we observed HOXD9 protein expression in human brain tumor tissues, including astrocytomas and glioblastomas. To investigate the role of HOXD9 in gliomas, we silenced its expression in the glioma cell line U87 using HOXD9-specific siRNA, and observed decreased cell proliferation, cell cycle arrest, and induction of apoptosis. It was suggested that HOXD9 contributes to both cell proliferation and/or cell survival. The HOXD9 gene was highly expressed in a side population (SP) of SK-MG-1 cells that was previously identified as an enriched-cell fraction of glioma cancer stem-like cells. HOXD9 siRNA treatment of SK-MG-1 SP cells resulted in reduced cell proliferation. Finally, we cultured human glioma cancer stem cells (GCSCs) from patient specimens found with high expression of HOXD9 in GCSCs compared with normal astrocyte cells and neural stem/progenitor cells (NSPCs). Our results suggest that HOXD9 may be a novel marker of GCSCs and cell proliferation and/or survival factor in gliomas and glioma cancer stem-like cells, and a potential therapeutic target. we analyzed the expression and function of HOXD9 in human gliomas and found high expression of HOXD9 in GCSCs. HOXD9 contributes to cell proliferation and/or survival in glioma cells and glioma cancer stem-like cells.

ORGANISM(S): Homo sapiens  

SUBMITTER: Cécile Thirant   Yumi Matsuzaki  Kazunari Yoshida  Hideyuki Saya  Shigeki Ohta  Yutaka Kawakami  Masanao Tabuse  Yohei Ohashi  Hideyuki Okano  Aya Misawa  Masahiro Toda  Takeshi Kawase  Raita Fukaya  Hérve Chneiweiss 

PROVIDER: E-GEOD-28618 | ArrayExpress | 2011-04-16

SECONDARY ACCESSION(S): GSE28618PRJNA138743

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications


BACKGROUND: HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. They also behave as oncogenes in some malignancies. RESULTS: In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. Using immunohistochemistry, we observed HOXD9 protein expression in human brain tumor tissues, including astrocytomas and  ...[more]

Similar Datasets

2011-04-16 | GSE28618 | GEO
| GSE103022 | GEO
2016-02-17 | PXD001778 | Pride
2018-10-22 | PXD008022 | Pride
| GSE76018 | GEO
| GSE58260 | GEO
2011-09-07 | E-MEXP-2910 | ArrayExpress
2011-09-07 | E-MEXP-2909 | ArrayExpress
2013-12-02 | E-GEOD-43674 | ArrayExpress
2010-06-21 | E-GEOD-18494 | ArrayExpress