Transcriptomics

Dataset Information

8

Cellular aging can be monitored by DNA-methylation changes at specific CpG sites


ABSTRACT: Long-term culture associated changes need to be considered for quality control of cell preparations – especially in cellular therapy. Here we describe a simple method to track cellular aging based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic signature can be used as a biomarker for various cell types to predict the state of cellular aging with regard to the number of passages or days of in vitro culture. 8 samples of human dermal fibroblasts. 4 samples of mesenchymal stem cells (MSC) from human adipose tissue.

ORGANISM(S): Homo sapiens  

SUBMITTER: Carmen M Koch   Sylvia Joussen  Anne Schellenberg  Qiong Lin  Martin Zenke  Wolfgang Wagner 

PROVIDER: E-GEOD-29661 | ArrayExpress | 2012-05-21

SECONDARY ACCESSION(S): GSE29661PRJNA141137

REPOSITORIES: GEO, ArrayExpress

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Publications

Monitoring of cellular senescence by DNA-methylation at specific CpG sites.

Koch Carmen M CM   Joussen Sylvia S   Schellenberg Anne A   Lin Qiong Q   Zenke Martin M   Wagner Wolfgang W  

Aging cell 20120119 2


Replicative senescence has fundamental implications on cell morphology, proliferation, and differentiation potential. Here, we describe a simple method to track long-term culture based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic senescence signature can be used as biomarker for various cell types to predict the state of cellular senescence with regard to the number of passages, population doublings, or days of in vitro culture. ...[more]

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