Transcriptomics

Dataset Information

4

The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset (RNAi)


ABSTRACT: The MOF-containing NSL complex binds to many but not all promoters of active genes and potentially contributes to their proper gene expression. It is currently unknown what determines whether an active gene is bound or not. Here, we provide evidence that the NSL complex primarily targets active promoters of most housekeeping genes. There, it co-localizes with the chromatin remodeler NURF and the histone methyltransferase Trithorax. Moreover, despite binding to most housekeeping genes, the NSL complex regulates only a subset of them, which are depleted for certain insulator binding-proteins and enriched for the core promoter motif “Ohler 5”. We suggest that the combination of general chromatin factors and core promoter motifs is predictive for whether a housekeeping gene is transcriptionally regulated by the NSL complex. Transcriptome expression profiling comparing NSL1 RNAi-depleted cells with control RNAi cells using Affymetrix microarrays.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Christian Feller   Peter Becker 

PROVIDER: E-GEOD-30990 | ArrayExpress | 2011-10-28

SECONDARY ACCESSION(S): GSE30990PRJNA154631

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset.

Feller Christian C   Prestel Matthias M   Hartmann Holger H   Straub Tobias T   Söding Johannes J   Becker Peter B PB  

Nucleic acids research 20111027 4


The MOF (males absent on the first)-containing NSL (non-specific lethal) complex binds to a subset of active promoters in Drosophila melanogaster and is thought to contribute to proper gene expression. The determinants that target NSL to specific promoters and the circumstances in which the complex engages in regulating transcription are currently unknown. Here, we show that the NSL complex primarily targets active promoters and in particular housekeeping genes, at which it colocalizes with the  ...[more]

Similar Datasets

2011-10-28 | E-GEOD-30991 | ArrayExpress
2011-10-28 | E-GEOD-30989 | ArrayExpress
2012-06-19 | E-MTAB-1084 | ArrayExpress
| GSE85504 | GEO
| GSE85503 | GEO
2014-06-03 | E-GEOD-51746 | ArrayExpress
| PRJNA154631 | ENA
| PRJNA146205 | ENA
| PRJNA154629 | ENA
2017-02-05 | E-MTAB-4455 | ArrayExpress