Dataset Information


Pre-miR-375_A549 vs. Pre-miR-NC#2 (Ambion) _A549

ABSTRACT: We investigated the functional significance of ASH1-inducible miR-375 in terms of biologic phenotypes of ASH1-positive lung cancer cells. To this end, we conducted genome-wide expression profiling analysis of miR-375-transfected A549 cells. Microarray analysis using a Whole Human Genome 4 x 44K Microarray G4112F (Agilent) was conducted to examine changes in expression of potential target genes of miR-375 by transfection of Pre-miR-375 or Pre-miR-NC#2 (Ambion) in A549 cells, which were then harvested at 12, 24, 48, and 96 hours after transfection.

ORGANISM(S): Homo sapiens  

SUBMITTER: Yasushi Yatabe   Yoshitaka Sekido  Hirotaka Osada  Ayumu Taguchi  Chinatsu Arima  Eri Nishikawa  Shuta Tomida  Osada Hirotaka  Kiyoshi Yanagisawa  Takashi Takahashi  Yasumasa Okazaki  Yukako Shimada  Shinya Toyokuni  Yoshio Tatematsu 

PROVIDER: E-GEOD-31566 | ArrayExpress | 2011-10-31



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Lung cancers with neuroendocrine (NE) features are often very aggressive but the underlying molecular mechanisms remain elusive. The transcription factor ASH1/ASCL1 is a master regulator of pulmonary NE cell development that is involved in the pathogenesis of lung cancers with NE features (NE-lung cancers). Here we report the definition of the microRNA miR-375 as a key downstream effector of ASH1 function in NE-lung cancer cells. miR-375 was markedly induced by ASH1 in lung cancer cells where it  ...[more]

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