Transcriptomics

Dataset Information

358

Identification of microRNAs association with Rheumatoid Arthritis Synovial Fibroblasts using the Human TNF Transgenic Mouse Model


ABSTRACT: OBJECTIVE: MicroRNAs (miRNAs, miRs), a class of small non-coding RNA molecules, are posttranscriptional regulators involved in a plethora of cellular functions and have been proposed as potential therapeutic targets in various diseases, including rheumatoid arthritis (RA). In this study, we sought to discover novel miR associations in synovial fibroblasts (SFs), a key cell type mediating RA pathogenesis, by performing miR expression profiling on cells isolated from the human TNF transgenic mouse model (TghuTNF or Tg197). METHODS: miR expression in SFs isolated from 8-week-old, fully diseased TghuTNF and WT littermate control mice were determined by deep sequencing of small RNAs and the arthritic profile was established by pairwise comparisons of the two groups. qRT-PCR analysis was utilised for profile validation purposes and miR quantitation in patient SFs. Dysregulated miR target genes and pathways were predicted via bioinformatic algorithms. Synovial Fibroblasts isolated from TghuTNF mice (2 x biological replicates) and control WT littermate mice (2 x biological replicates)

ORGANISM(S): Mus musculus  

SUBMITTER: Niki Karagianni   George Kollias  Ioannis Pandis  Jiannis Ragoussis  Artemis G Hatzigeorgiou  Carme Camps  Maria C Denis  Martin Reczko 

PROVIDER: E-GEOD-31667 | ArrayExpress | 2012-05-13

SECONDARY ACCESSION(S): SRP007941GSE31667PRJNA145387

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Identification of microRNA-221/222 and microRNA-323-3p association with rheumatoid arthritis via predictions using the human tumour necrosis factor transgenic mouse model.

Pandis Ioannis I   Ospelt Caroline C   Karagianni Niki N   Denis Maria C MC   Reczko Martin M   Camps Carme C   Hatzigeorgiou Artemis G AG   Ragoussis Jiannis J   Gay Steffen S   Kollias George G  

Annals of the rheumatic diseases 20120505 10


To identify novel microRNA (miR) associations in synovial fibroblasts (SF), by performing miR expression profiling on cells isolated from the human tumour necrosis factor (TNF) transgenic mouse model (TghuTNF, Tg197) and patients biopsies.miR expression in SF from TghuTNF and wild-type (WT) control mice were determined by miR deep sequencing (miR-seq) and the arthritic profile was established by pairwise comparisons. Quantitative PCR analysis was utilised for profile validation, miR and gene qua  ...[more]

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