Dataset Information


Gene expression in Drosophila Poly mutant and wild-type

ABSTRACT: The Poly mutant was identified as a previously uncharacterised, but essential gene in Drosphila. Loss of Poly reults in lethality at the third larval instar stage, but only after a stage of extreme larval longevity. We aimed to assess the consequences of loss of the Poly gene on glocal gene expression by microarray analysis. Metabolism is altered in Poly mutants and here we find altered gene expression in insulin signaling pathways. Four biological replicates each of Poly mutant and wild-type larvae were used to assess gene expression on FL002 microarrays (GPL5135) . These data reveal that Poly is a mediatior of insulin receptor/TOR signaling. Four biological replicates of poly mutant and wild-type Drosophila. Two arrays had wild-type in Cy5 channel and poly in Cy3 channel, the other two were reversed.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Donald R Dunbar   Donald Dunbar  Margarete M Heck  Ekin Bolukbasi 

PROVIDER: E-GEOD-32637 | ArrayExpress | 2012-01-17



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Drosophila poly suggests a novel role for the Elongator complex in insulin receptor-target of rapamycin signalling.

Bolukbasi Ekin E   Vass Sharron S   Cobbe Neville N   Nelson Bryce B   Simossis Victor V   Dunbar Donald R DR   Heck Margarete M S MM  

Open biology 20120101 1

Multi-cellular organisms need to successfully link cell growth and metabolism to environmental cues during development. Insulin receptor-target of rapamycin (InR-TOR) signalling is a highly conserved pathway that mediates this link. Herein, we describe poly, an essential gene in Drosophila that mediates InR-TOR signalling. Loss of poly results in lethality at the third instar larval stage, but only after a stage of extreme larval longevity. Analysis in Drosophila demonstrates that Poly and InR i  ...[more]

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