Transcriptomics

Dataset Information

4

SU(HW)-HB.S2.LacZ_RNAi


ABSTRACT: modENCODE_submission_3719 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The goal of these experiments are to validate and confirm the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. To do this, we are using RNAi to deplete individual non-histone chromosomal proteins in Drosophila BG3 and S2 tissue culture cells, and then using antibodies to perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. Comparison of a protein factor's binding profiles before and after depletion will increase the confidence of our predictions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody SU(HW)-HB (target is SU(HW)); dsRNA (RNAi_reagent) Fly_LacZ_RNAi&oldid=39667

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Nicole Riddle   Gary Karpen  DCC modENCODE  Vincenzo Pirrotta  Andrey Gortchakov  Art Alekseyenko  Michael Tolstorukov  Greg Shanower  Sarah Elgin  Aki Minoda  Mitzi Kuroda  Cameron Kennedy  Yuri Schwartz  Peter Park  Peter Kharchenko 

PROVIDER: E-GEOD-32813 | ArrayExpress | 2011-10-12

SECONDARY ACCESSION(S): GSE32813

REPOSITORIES: GEO, ArrayExpress

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