Transcriptomics

Dataset Information

2

Genome-wide analysis of gene expression by compound 1 treatment [HBEC_6_12hr]


ABSTRACT: Analysis of cellular response to DHODH inhibition at gene expression level. The NS1 protein of influenza virus is a major virulence factor essential for virus replication as it re-directs the host cell to promote viral protein expression. NS1 inhibits cellular mRNA processing and export, down-regulating host gene expression and enhancing viral gene expression. We report here the identification of a non-toxic quinoline carboxylic acid that reverts the inhibition of mRNA nuclear export by NS1, in the absence or presence of virus. This quinoline carboxylic acid directly inhibited dihydroorotate dehydrogenase (DHODH), a host enzyme required for *de novo* pyrimidine biosynthesis, and partially reduced pyrimidine levels. This effect induced NXF1 expression, which promoted mRNA nuclear export in the presence of NS1. The release of NS1-mediated mRNA export block by DHODH inhibition also occurred in the presence of VSV M protein, another viral inhibitor of mRNA export. This reversal of mRNA export block allowed expression of antiviral factors. Thus, pyrimidines play a necessary role in the inhibition of mRNA nuclear export by virulence factors. Total RNA obtained from HBEC cells subjected to compound 1/compound 1-14 treatment compared to DMSO treatment.

ORGANISM(S): Homo sapiens  

SUBMITTER: liang zhang   Beatriz Fontoura  Liang Zhang 

PROVIDER: E-GEOD-35116 | ArrayExpress | 2012-01-17

SECONDARY ACCESSION(S): GSE35116PRJNA156013

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2012-01-17 | E-GEOD-35115 | ArrayExpress
2012-01-17 | E-GEOD-35113 | ArrayExpress
2012-01-17 | E-GEOD-35114 | ArrayExpress
2012-01-17 | E-GEOD-35112 | ArrayExpress
| GSE35112 | GEO
| GSE121266 | GEO
| GSE87577 | GEO
2013-06-01 | E-GEOD-42531 | ArrayExpress
| GSE82241 | GEO
| GSE67338 | GEO