Dataset Information


S phase and HU profiles in wild-type and mutant cells

ABSTRACT: Total S phase was measured for wild-type cells undergoing meiS and mitS. Early replication origins were mapped in mitS in wild-type cells, and in meiS for wild-type, sml1 delete, rec8 delete and spo11 delete cells. Comparative genome hybridization was used to measure the kinetics and final extent of DNA replication in wild-type and mutant cells. First, S phase profiles were produced from wild-type cells undergoing pre-mitotic S phase (mitS) and pre-meiotic S phase (meiS) to measure the kinetics of DNA replication in the two S phases. For these experiments, a pool of samples collected throught out all of S phase were labeled and hybridized to a microarray together with a control G1 DNA sample. Second, early replication origins were mapped by exposing cells to levels of HU that strongly reduced DNA replication. Early replication was compared for wild-type cells in mitS to wild-type, sml1, rec8 and spo11 delete strain undergoing meiS. In these experiment, samples were collected after 4 hours exposure to HU. Total genomic DNA was isolated, labeled and hybridized to a microarray together with a control G1 DNA sample. A control G1 vs. G1 sample was prepared to measure the level of noise inherent in the CGH technique.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Andreas Hochwagen   Hannah G Blitzblau  Clara S Chan  Stephen P Bell 

PROVIDER: E-GEOD-35662 | ArrayExpress | 2012-06-18



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Separation of DNA replication from the assembly of break-competent meiotic chromosomes.

Blitzblau Hannah G HG   Chan Clara S CS   Hochwagen Andreas A   Bell Stephen P SP  

PLoS genetics 20120517 5

The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-meiotic S phase (meiS) and the assembly of meiotic chromosome axes, remain poorly defined. We have used genome-wide approaches in Saccharomyces cerevisiae to measure the kinetics of pre-meiotic DNA rep  ...[more]

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