Genomics

Dataset Information

299

Global Mapping at the Core of the Arabidopsis Circadian Clock Defines the Network Structure of the Oscillator


ABSTRACT: In many organisms, the circadian clock is composed of functionally coupled morning and evening oscillators that regulate the bouts of dawn and dusk activity. In Arabidopsis, oscillator coupling relies on a core loop in which the evening oscillator component TOC1 was proposed to activate a subset of morning-expressed oscillator genes. Our systems-biological approach overturns the current view of the Arabidopsis circadian clock showing that TOC1 does not function as an activator but as a timely-controlled general repressor of morning and evening oscillator components. Repression occurs through rhythmic binding to the promoters of all oscillator genes, suggesting a previously unexpected direct connection between the morning and evening loops. Examination of TOC1 genome-wide binding using TOC1 Minigene (TMG) seedlings expressing the genomic fragment of TOC1 fused to the Yellow Fluorescent Protein in a toc1-2 mutant background (TMG-YFP/toc1-2 seedlings) grown under LD cycles (12h light:12h dark).

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Jose Luis Riechmann   Igor Antoshechkin  Paloma Mas  José L Riechmann 

PROVIDER: E-GEOD-35952 | ArrayExpress | 2012-03-08

SECONDARY ACCESSION(S): GSE35952SRP010999PRJNA151987

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Mapping the core of the Arabidopsis circadian clock defines the network structure of the oscillator.

Huang W W   Pérez-García P P   Pokhilko A A   Millar A J AJ   Antoshechkin I I   Riechmann J L JL   Mas P P  

Science (New York, N.Y.) 20120308 6077


In many organisms, the circadian clock is composed of functionally coupled morning and evening oscillators. In Arabidopsis, oscillator coupling relies on a core loop in which the evening oscillator component TIMING OF CAB EXPRESSION 1 (TOC1) was proposed to activate a subset of morning-expressed oscillator genes. Here, we show that TOC1 does not function as an activator but rather as a general repressor of oscillator gene expression. Repression occurs through TOC1 rhythmic association to the pro  ...[more]

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