Transcriptomics

Dataset Information

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Lung Natural Helper cells


ABSTRACT: Natural Helper cells constitute a unique lineage of Th2-cytokine producting innate lymphocytes, here we characterize the gene expression profile of non-stimulated or PMA/ionomycin-stimulated Natural Helper cells from naive C57Bl/6 mouse lungs. We used microarrays to detail the gene expression profile of stimulated and unstimulated lung Natural Helper cells in mice. Lineage(-)Sca-1(+)c-Kit(-/low)CD127(+)CD25(+) Lung Natural Helper cells were purified from naive 6-8 week old B6 mice by FACS. RNA was extracted immediately from un-stimulated Natural Helper cells after FACS. Stimulated Natural Helper cells were cultured with PMA/ionomycin for 3 days followed by RNA extraction.

ORGANISM(S): Mus musculus  

SUBMITTER: Timotheus You Fu Halim   Timotheus Y Halim  Fumio Takei 

PROVIDER: E-GEOD-36057 | ArrayExpress | 2012-02-24

SECONDARY ACCESSION(S): GSE36057PRJNA151675

REPOSITORIES: GEO, ArrayExpress

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