Transcriptomics

Dataset Information

2

In vivo gene expression data from wild type and CTLA-4 KO 5C.C7 T cells


ABSTRACT: CTLA-4 is thought to inhibit effector T cells both intrinsically, by competing with CD28 for B7 ligands, and extrinsically, through the action of regulatory T cells. We studied in vivo responses of normal and CTLA-4-deficient antigen-specific murine effector CD4+ T cells. In order to do these studies in a physiological model of immunity to foreign antigen, we transferred small numbers of congenically marked RAG2-deficient 5C.C7 T cells with either a normal or knockout allele of CTLA-4 into normal syngeneic B10.A recipient mice. The T cells were then activated by immunization with MCC peptide and LPS. To look for transcriptional signatures of negative regulation of T cell responses by CTLA-4, we used microarray analysis to compare transcripts in wild type and CTLA-4 KO 5C.C7 T cells four days after immunization. This is the first instance in which differences are observed in extent of accumulation of wild type and CTLA-4 KO 5C.C7 T cells. To compare the gene expression profile between wild type and CTLA-4 KO adoptively transferred T cells 4 days after immunization.

ORGANISM(S): Mus musculus  

SUBMITTER: James P Allison   Emily Corse  Jeffrey Zhao 

PROVIDER: E-GEOD-37563 | ArrayExpress | 2012-09-05

SECONDARY ACCESSION(S): GSE37563PRJNA161865

REPOSITORIES: GEO, ArrayExpress

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Publications

Cutting edge: CTLA-4 on effector T cells inhibits in trans.

Corse Emily E   Allison James P JP  

Journal of immunology (Baltimore, Md. : 1950) 20120702 3


CTLA-4 is thought to inhibit effector T cells both intrinsically, by competing with CD28 for B7 ligands, and extrinsically, through the action of regulatory T cells (Tregs). We studied in vivo responses of normal and CTLA-4-deficient Ag-specific murine effector CD4(+) T cells. We directly demonstrate that effector T cell-restricted CTLA-4 inhibits T cell responses in a cell-extrinsic manner. Cotransfer experiments show that CTLA-4 on normal effector CD4(+) T cells completely abrogates the dramat  ...[more]

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