Transcriptomics

Dataset Information

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Notch signaling/MyoD double deficient embryonic muscle progenitors


ABSTRACT: Skeletal muscle growth and regeneration rely on myogenic progenitor and satellite cells, the stem cells of postnatal muscle. Elimination of Notch signals during mouse development results in premature differentiation of myogenic progenitors and formation of very small muscle groups. Here we show that this drastic effect is rescued by mutation of the muscle differentiation factor MyoD. However, rescued myogenic progenitors do not assume a satellite cell position and contribute poorly to myofiber growth. The disrupted homing is due to a deficit in basal lamina assembly around emerging satellite cells and to their impaired adhesion to myofibers. On a molecular level, emerging satellite deregulate the expression of basal lamina components and adhesion molecules like integrin a7, collagen XVIIIa1, Megf10 and Mcam. We conclude that Notch signals control homing of satellite cells, stimulating them to contribute to their own microenvironment and to adhere to myofibers. Gene expression analysis using total RNA from FACS-isolated Vcam-1+/CD31-/CD45-/Sca1- embryonic muscle progenitor cells from E17.5 back muscle tissue of MyoD-/-, Pax3cre/+;Rbpjflox/flox;MyoD-/- and Pax3cre/+;DnMamlflox/flox;MyoD-/- mice.

ORGANISM(S): Mus musculus  

SUBMITTER: Maciej T Czajkowski   Elena Vasyutina  Hans-Peter Rahn  Joscha Griger  Bettina Purfürst  Claudia Rassek  Dominique Bröhl  Carmen Birchmeier  Hagen Wende 

PROVIDER: E-GEOD-39379 | ArrayExpress | 2012-12-31

SECONDARY ACCESSION(S): GSE39379PRJNA170741

REPOSITORIES: GEO, ArrayExpress

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Publications

Colonization of the satellite cell niche by skeletal muscle progenitor cells depends on Notch signals.

Bröhl Dominique D   Vasyutina Elena E   Czajkowski Maciej T MT   Griger Joscha J   Rassek Claudia C   Rahn Hans-Peter HP   Purfürst Bettina B   Wende Hagen H   Birchmeier Carmen C  

Developmental cell 20120830 3


Skeletal muscle growth and regeneration rely on myogenic progenitor and satellite cells, the stem cells of postnatal muscle. Elimination of Notch signals during mouse development results in premature differentiation of myogenic progenitors and formation of very small muscle groups. Here we show that this drastic effect is rescued by mutation of the muscle differentiation factor MyoD. However, rescued myogenic progenitors do not assume a satellite cell position and contribute poorly to myofiber g  ...[more]

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