Transcriptomics

Dataset Information

62

RUNX1 reshapes the epigenetic landscape at the onset of hematopoiesis


ABSTRACT: Cell fate decisions during hematopoiesis are governed by lineage-specific transcription factors, such as RUNX1, SCL/TAL1, FLI1 and C/EBP family members. In order to gain insight about how these transcription factors regulate the activation of hematopoietic genes during embryonic development, we measured the genome-wide dynamics of transcription factor assembly on their target genes during the RUNX1-dependent transition from hemogenic endothelium to hematopoietic progenitors. Using a RUNX1-/- embryonic stem cell differentiation model expressing an inducible RUNX1 gene, we show that in the absence of RUNX1, SCL/TAL1, FLI1 and C/EBP-beta prime hematopoietic genes and that this early priming is required for correct temporal expression of the myeloid master regulator PU.1 and its downstream targets. After induction, RUNX1 binds to numerous new sites, initiating a local increase of histone acetylation and rapid global alterations in the binding patterns of SCL/TAL1 and FLI1. The acquisition of hematopoietic fate controlled by RUNX1 therefore does not represent the establishment of a new regulatory layer on top of a pre-existing hemogenic endothelium program but instead entails global reorganization of lineage-specific transcription factor assemblies. Microarray expression data obtained from differentiating murine hematopoietic cells, 3 independent biological replicates (measured twice) from iRUNX1 culture -/+DOX induction

ORGANISM(S): Mus musculus  

SUBMITTER: Monika Lichtinger   Salam A Assi  Rebecca Hannah  Salam Adli Assi  Mengchu Wu  Valerie Kouskoff  Georges Lacaud  Berthold Göttgens  Michael Lie-A-Ling  Deborah Clarke  Daniel G Tenen  MS Vijayabaskar  Richard Ingram  Constanze Bonifer  Dorothee Müller  Laura Noailles  David R Westhead 

PROVIDER: E-GEOD-39662 | ArrayExpress | 2013-02-11

SECONDARY ACCESSION(S): GSE39662PRJNA171352

REPOSITORIES: GEO, ArrayExpress

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Publications


Cell fate decisions during haematopoiesis are governed by lineage-specific transcription factors, such as RUNX1, SCL/TAL1, FLI1 and C/EBP family members. To gain insight into how these transcription factors regulate the activation of haematopoietic genes during embryonic development, we measured the genome-wide dynamics of transcription factor assembly on their target genes during the RUNX1-dependent transition from haemogenic endothelium (HE) to haematopoietic progenitors. Using a Runx1-/- embr  ...[more]

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