Browse
Submit Data
Databases
Help
Login

Dataset Information

7 Views

1 Connections

0 Citations

0 Reanalyses

6 Downloads

Omics score: 2

Acute hepatic transcriptional response in male mice following oral exposure to benzo(a)pyrene

ABSTRACT: Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male B6C3F1 mice were exposed to four doses of benzo(a)pyrene or vehicle control for three days and sacrificed 4 or 24 hours after the final exposure. This experiment examined the hepatic transcriptional response of male mice exposed to BaP for 3 days at four different doses, including D1 (300 mg/kg BW/day), D2 (150 mg/kg BW/day), D3 (50 mg/kg BW/day) and D4 (5 mg/kg BW/day), and one control. Each dose group was further examined at 2 time points, 4 hours and 24 hours, following the final exposure. Each dose group and time point had 4-5 biological replicates. There were a total 46 samples (arrays) included in the final analysis using a two-colour reference design.

ORGANISM(S): Mus musculus

SUBMITTER: Sabina Halappanavar Mirjam Luijten France Lemieux Nikolai L Chepelev Julie A Bourdon Julie K Buick Sarah Labib Kelly Jackson Andrew Williams Amal I Malik Ivy D Moffat Carole L Yauk Byron Kuo

PROVIDER: E-GEOD-43633 | ArrayExpress | 2015-07-01

SECONDARY ACCESSION(S): GSE43633PRJNA186868

REPOSITORIES: GEO, ArrayExpress

ACCESS DATA

Json Xml

Similar Datasets

Sub-chronic forestomach transcriptional response in adult male MutaTMMouse following oral exposure to benzo(a)pyrene

Project description:Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male MutaTMMouse were exposed to three doses of benzo(a)pyrene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. This experiment examined the forestomach transcriptional response of male mice exposed to BaP for 28 days at three different doses, including D1 (25 mg/kg BW/day), D2 (50 mg/kg BW/day), and D3 (75 mg/kg BW/day) and vehicle control. Each dose group was examined 72 hours following the final exposure. Each dose group and time point had 4-5 biological replicates. There were a total 17 samples (arrays) included in the final analysis using a two-colour reference design.

2013-09-27 | E-GEOD-43438 | ArrayExpress

Sub-chronic pulmonary transcriptional response to a mixture of 8 polycyclic aromatic hydrocarbons in adult male MutaTMMouse.

Project description:Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. The mice in this study were exposed to a mixture of 8 PAHs, benzo(a)pyrene, benz(a)anthracene, benzo(b)fluoranthene, benzo(ghi)perylene, benzo(k)fluoranthene, chrysene, dibenz(ah)anthracene, and indeno(123,cd)pyrene. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to this PAH mixture in the lung tissue. Adult male MutaTMMouse were exposed to three doses of the mixture of 8 PAHs or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. Overall design: This experiment examined the pulmonary transcriptional response of male mice exposed to a 8PAH mixture for 28 days at three different doses, including D1 (15 mg BaP equivalents/kg-bw/day), D2 (30 mg BaP equivalents/kg-bw/day), and D3 (60 mg BaP equivalents/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 4-5 biological replicates. There were a total of 19 samples (arrays) from the lung included in the final analysis using a two-colour reference design.

| GSE87689 | GEO

Sub-chronic pulmonary transcriptional response to a mixture of 4 PAHs in adult male MutaTMMouse.

Project description:Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. The mice in this study were exposed to a mixture of 4 PAHs, benzo(a)pyrene, benz(a)anthracene, benzo(b)fluoranthene, and chrysene. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to this PAH mixture in the lung tissue. Adult male MutaTMMouse were exposed to three doses of the mixture of 4 PAHs or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. Overall design: This experiment examined the pulmonary transcriptional response of male mice exposed to a 4PAH mixture for 28 days at three different doses, including D1 (12.5 mg BaP equivalents/kg-bw/day), D2 (25 mg BaP equivalents/kg-bw/day), and D3 (50 mg BaP equivalents/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 4-5 biological replicates. There were a total of 18 samples (arrays) from the lung included in the final analysis using a two-colour reference design.

| GSE87688 | GEO

Gene expression profiles for onset and progression of Cyclosporin A-induced cholestasis in C57BL/6 mice

Project description:Mechanism-based toxicogenomics (tgx) is used as a tool to identify markers reflective of the onset and progression of cholestasis in C57BL/6 mice using Cyclosporin A (CsA) as a model compound. Critical doses for tgx analysis were derived from a dose range finding study in which increase of serum cholesterol, total bile acids, and total bilirubin as well as induction of hepatocyte vacuolization 25 days upon repeated CsA administration through oral gavage were considered as critical effects. For tgx analysis to find early markers, livers of mice repeatedly treated with 3 mg/kg BW, 8.9 mg/kg BW, and 26.7 mg/kg BW for one, four, and eleven days were collected. 60 samples are analyzed; per treatment duration (1, 4, 11 days), time-matched vehicle (olive oil) controls and three dose groups (3, 8.9, 26.7 mg/kg BW) were included; each group consisted of 5 replicates; 3 arrays were excluded, 2 because of quality control restrictions, 1 because of outlier properties. 2 that failed QC are omitted. Final data consists of 58 CEL files.

2013-07-11 | E-GEOD-31540 | ArrayExpress

Cell death and regenerative proliferation pathways in hepatocellular carcinoma: The effect of furan on B6C3F1 mouse global gene expression in liver

Project description:Furan is a mouse and rat hepatocarcinogen. We sought to determine the dose-dependent changes in gene expression upon exposure of B6C3F1 mice to furan in order to better understand furan’s mode of action. Adult female B6C3F1 mice were exposed to 1, 2, 4 or 8 mg/kg bw furan or vehicle control (corn oil) for three weeks and sacrificed four hours after the final exposure. In this study we examined the transcriptional response in liver tissue of female B6C3F1 mice exposed to furan for 3 weeks at four different doses: 1, 2, 4 or 8 mg/kg bw furan (or vehicle control) and sacrificed four hours after the final exposure. Each dose group had 4-5 biological replicates. There were a total of 25 samples included in the final analysis. We used a two-colour reference design and SurePrint G3 Mouse GE 8x60K microarrays (Agilent).

2014-01-10 | E-GEOD-48644 | ArrayExpress

Sub-chronic transcriptional response in adult male MutaTMMouse following oral exposure to benzo(ghi)perylene in lung, liver, and forestomach tissues [BghiP]

Project description:Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. benzo(ghi)perylene is one such compound. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to benz(a)anthracene in the lung, liver, and forestomach tissues. Adult male MutaTMMouse were exposed to three doses of benzo(ghi)perylene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. Overall design: This experiment examined the pulmonary, hepatic, and forestomach transcriptional response of male mice exposed to benzo(ghi)perylene for 28 days at three different doses, including D1 (6.25 mg/kg-bw/day), D2 (12.5 mg/kg-bw/day), and D3 (25 mg/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 4-5 biological replicates. There were a total 20, 20, and 20 samples (arrays) from the lung, liver, and forestomach, respectively, included in the final analysis using a two-colour reference design.

| GSE51316 | GEO

Sub-chronic transcriptional response in adult male MutaTMMouse following oral exposure to benzo(k)fluoranthene in lung, liver, and forestomach tissues [BkF]

Project description:Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. Benzo(k)fluoranthen is one such compound. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to benz(a)anthracene in the lung, liver, and forestomach tissues. Adult male MutaTMMouse were exposed to three doses of benzo(k)fluoranthene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. Overall design: This experiment examined the pulmonary, hepatic, and forestomach transcriptional response of male mice exposed to benzo(k)fluoranthene for 28 days at three different doses, including D1 (25 mg/kg-bw/day), D2 (50 mg/kg-bw/day), and D3 (100 mg/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 2-4 biological replicates. There were a total 15, 13, and 14 samples (arrays) from the lung, liver, and forestomach, respectively, included in the final analysis using a two-colour reference design.

| GSE51317 | GEO

Sub-chronic transcriptional response in adult male MutaTMMouse following oral exposure to benzo(b)fluoranthene in lung, liver, and forestomach tissues [BbF]

Project description:Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. Benzo(b)fluoranthene is one such compound. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to benz(a)anthracene in the lung, liver, and forestomach tissues. Adult male MutaTMMouse were exposed to three doses of Benzo(b)fluoranthene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. Overall design: This experiment examined the pulmonary, hepatic, and forestomach transcriptional response of male mice exposed to benz(a)anthracene for 28 days at three different doses, including D1 (25 mg/kg-bw/day), D2 (50 mg/kg-bw/day), and D3 (100 mg/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 4-5 biological replicates. There were a total 19, 20, and 20 samples (arrays) from the lung, liver, and forestomach, respectively, included in the final analysis using a two-colour reference design.

| GSE51315 | GEO

Transcription profiling of mouse livers which have been exposed to dechlorane plus by gavage

Project description:Five-week-old male mice (Mus musculus, ICR) were exposed to dechlorane plus for 10 days. A total of tweenty-four mice were randomly assigned to control and three DP-treated groups. Six mice were applied in every group. For control group, corn oil was given to mice by gavage daily. For three DP-treated groups, mice received a daily dose of DP in corn oil at 500 mg DP /kg bw, 2000 mg DP /kg bw and 5000 mg DP /kg bw, respectively. After exposure, mice were anaesthetized under isoflurane followed by exsanguination. Livers were removed, and hepatic RAN for each mouse were immediately extract. The gene expression profiles for control and three DP-treated groups were determined by the GeneChip? Mouse Exon 1.0 ST arrays. the biological significances of differentially expressed genes (DEGs) were analyzed based on Molecule Annotation System 3.0 (MAS3.0, http://bioinfo.capitalbio.com/mas/). The microarray analysis of this study provide novel insight regarding toxicological effects and mechanism of DP at the transcriptome level

2012-09-05 | E-MEXP-3707 | ArrayExpress

Sub-chronic transcriptional response in adult male MutaTMMouse following oral exposure to indeno(123,cd)pyrene in lung, liver, and forestomach tissues [IP]

Project description:Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. Indeno(123,cd)pyrenee is one such compound. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to benz(a)anthracene in the lung, liver, and forestomach tissues. Adult male MutaTMMouse were exposed to three doses of indeno(123,cd)pyrene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure. Overall design: This experiment examined the pulmonary, hepatic, and forestomach transcriptional response of male mice exposed to indeno(123,cd)pyrene for 28 days at three different doses, including D1 (12.5 mg/kg-bw/day), D2 (25 mg/kg-bw/day), and D3 (50 mg/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 4-5 biological replicates. There were a total 20, 19, and 20 samples (arrays) from the lung, liver, and forestomach, respectively, included in the final analysis using a two-colour reference design.

| GSE51320 | GEO

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
GPMDB
JPOST Repository

EGA
EVA
ENA
LINCS
PAXDB

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels

ArrayExpress
dbGaP
ExpressionAtlas
GEO

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data