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Acute hepatic transcriptional response in male mice following oral exposure to benzo(a)pyrene

ABSTRACT: Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male B6C3F1 mice were exposed to four doses of benzo(a)pyrene or vehicle control for three days and sacrificed 4 or 24 hours after the final exposure. This experiment examined the hepatic transcriptional response of male mice exposed to BaP for 3 days at four different doses, including D1 (300 mg/kg BW/day), D2 (150 mg/kg BW/day), D3 (50 mg/kg BW/day) and D4 (5 mg/kg BW/day), and one control. Each dose group was further examined at 2 time points, 4 hours and 24 hours, following the final exposure. Each dose group and time point had 4-5 biological replicates. There were a total 46 samples (arrays) included in the final analysis using a two-colour reference design.

ORGANISM(S): Mus musculus

SUBMITTER: Sabina Halappanavar Mirjam Luijten France Lemieux Nikolai L Chepelev Julie A Bourdon Julie K Buick Sarah Labib Kelly Jackson Andrew Williams Amal I Malik Ivy D Moffat Carole L Yauk Byron Kuo

PROVIDER: E-GEOD-43633 | ArrayExpress | 2015-07-01

SECONDARY ACCESSION(S): GSE43633PRJNA186868

REPOSITORIES: GEO, ArrayExpress

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