Genome-wide analysis of MAOA on Norepinphrine induced HCC gene expression
ABSTRACT: Gene analysis of HCC cells transfected with Lenti-vector, Lenti-MAOA and stimulated with Norepinephrine Total mRNA obtained from SMMC-7721 cells transfected with Lenti-vector, Lenti-MAOA and stimulated with Norepinephrine
Project description:We performed a genome wide transcription profile analysis to determine the expression alterations between control and the POH1 siRNAs transfected SMMC-7721 cells The liver cancer cell line SMMC-7721 transfected with either the control or POH1 siRNAs were subjected to a genome wide transcription profile analysis through Human U133 Puls 2.0 (Affymetrix) microarray
Project description:The goals of this study are to analyze the transcriptome profiling (RNA-seq) after PBX3 overexpression in SMMC-7721 cells. Overall design: SMMC-7721 cells were transfected by PBX3 expression lentivirus and blank lentivirus as control. Total RNA was extraced by Qiagen kit and the mRNA profiles were generated by deep sequencing, in duplicate, using Illumina HiSeq 2000.
Project description:Our study have demonstrated LINC01138 acts as an oncogenic driver that promotes cell proliferation, tumourigenicity, tumour invasion and metastasis through physically interacting with Arginine Methyltransferase 5 (PRMT5) in HCC cells. In order to investigate the related signaling pathways regulated by LINC01138 or PRMT5, we performed the unbiased transcriptome profiling using high-throughput RNA sequencing in SMMC-7721 cells transfected with si-LINC01138 or si-PRMT5. Here we showed the LINC01138/PRMT5 axis is an ideal therapeutic target for HCC treatment. Overall design: Illuminate the related signaling pathways and biological processes regulated by LINC01138 or PRMT5 in human liver cancer SMMC-7721 cells through high-throughput sequencing data.
Project description:We report the high-throughput profiling of PBX3-binding sites under liver cancer stem cell reprogramming driven by PBX3 overexpression. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide PBX3-binding maps of SMMC-7721 cells. This study provides a prediction of regulated genes by the PBX3. Overall design: Examination of PBX3-binding sites in SMMC-7721 cell with overexpression PBX3. The fragmented DNA was precipitated with a PBX3 antibody.
Project description:lncRNA-PRAL induced transcriptional changes in SMMC-7721 Overall design: Two-condition experiment, control (AV-CON) vs lncRNA-PRAL expression (AV-PRAL). Each has 3 biological repeats.
Project description:In our present study, we found that lncRNA-hPVT1 could promote HCC cell proliferation. We want to know what were the target genes of lncRNA-hPVT1. So we constructed the hPVT1-overexpressed SMMC-771 cells and observed the mRNA profile in hPVT1-overexpressed and control SMMC-771 cells. lncRNA-hPVT1 induced transcriptional changes in SMMC-7721. Two-condition experiment, control (LV-Control) vs lncRNA-hPVT1 expression (LV-hPVT1 Clone2 ). Each has 3 biological repeats.