Dataset Information


Gene expression profiling of pancreatic cancer cell line BxPC-3 induced by PAR-2 AP

ABSTRACT: Protease-activated receptor-2 (PAR-2), a G protein-coupled receptor activated by trypsin and coagulant factors, plays broad spectrum of physiological and pathological roles especially in cancer development. In this study, we used PAR-2 activating peptide to mimic the action of trypsin to trigger PAR-2 signaling pathway and effects of PAR-2 activation on gene expression in human pancreatic cancer cell line BxPC-3 investigated by microarray analysis. Through DAVID bioinformatic resources, we observed that activated PAR-2-mediated genes are summarized to two different pathways, renal cell carcinoma and NFkB pathway. In renal cell carcinoma pathway, activated PAR-2 dysregulated hypoxia-inducible factors and its target genes, including glucose transporter 1 (GLUT1), transforming growth factor-b (TGF-b) and vascular endothelial growth factor-A (VEGF-A). In addition, activated PAR-2 induced MAPK signaling and transcriptional factors, such as JUN, MAP2K1 and ETS1. The regulation of these genes by PAR-2 assumed that PAR-2 signaling was associated with cancer progression. On the other hand, activated PAR-2 upregulated interleukin-1b (IL-1b) and toll-like receptor 4 (TLR4) related with NFkB activation, which indicated that PAR-2 signaling may cause cancer-related inflammation. In conclusion, PAR-2 may be a factor to regulate cancer progression and inflammation. Two-condition experiment, control cells vs PAR-2 AP-treated cells.

ORGANISM(S): Homo sapiens  

SUBMITTER: An-Chi Tsai   Li-Hsun Chang  Chin-Yu Lai  Shiow-Lin Pan  Che-Ming Teng 

PROVIDER: E-GEOD-44827 | ArrayExpress | 2013-04-30



Similar Datasets

| GSE44827 | GEO
2013-10-01 | E-GEOD-49560 | ArrayExpress
2013-12-01 | E-GEOD-48468 | ArrayExpress
2015-11-10 | E-GEOD-74829 | ArrayExpress
2014-01-10 | E-GEOD-53953 | ArrayExpress
2015-08-01 | E-GEOD-48961 | ArrayExpress
2014-11-17 | E-GEOD-52365 | ArrayExpress
2016-06-30 | E-GEOD-76613 | ArrayExpress
2013-05-30 | E-GEOD-47500 | ArrayExpress
2015-07-01 | E-GEOD-70409 | ArrayExpress