Transcriptomics

Dataset Information

14

Cell host-response to infection with novel human coronavirus EMC predict potential antivirals and important differences with SARS-coronavirus.


ABSTRACT: Differential expression was determined in Calu-3 cells between mock infected and infection with either Human coronavirus EMC and SARS coronavirus at different times post infection. Calu-3 2B4 cells were infected with Human Coronavirus EMC 2012 (HCoV-EMC) or mock infected. Samples were collected 0, 3, 7, 12, 18 and 24 hpi. There are 3 mock and 3 infected replicates for each time point, except for 12 hpi for which there are only 2 infected replicates (one replicate did not pass RNA quality check). There were no mock sampes at 18 hpi, and therefore infected samples at 18 hpi were compared with mocks at 24 hpi. For direct comparison with SARS-CoV infected cells, raw data from HCoV-EMC experiments were quantile normalized together with the SARS-CoV dataset (GEO Series accession number GSE33267).

ORGANISM(S): Homo sapiens  

SUBMITTER: Michael G Katze   Ralph S Baric  Boyd L Yount  Laurence Josset  Vineet D Menachery  Lisa E Gralinski  Victoria S Carter  Michael Katze  Pavel Sova  Rachel L Graham  Sudhakar Agnihothram 

PROVIDER: E-GEOD-45042 | ArrayExpress | 2013-05-03

SECONDARY ACCESSION(S): GSE45042PRJNA192892

REPOSITORIES: GEO, ArrayExpress

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Publications

Cell host response to infection with novel human coronavirus EMC predicts potential antivirals and important differences with SARS coronavirus.

Josset Laurence L   Menachery Vineet D VD   Gralinski Lisa E LE   Agnihothram Sudhakar S   Sova Pavel P   Carter Victoria S VS   Yount Boyd L BL   Graham Rachel L RL   Baric Ralph S RS   Katze Michael G MG  

mBio 20130430 3


A novel human coronavirus (HCoV-EMC) was recently identified in the Middle East as the causative agent of a severe acute respiratory syndrome (SARS) resembling the illness caused by SARS coronavirus (SARS-CoV). Although derived from the CoV family, the two viruses are genetically distinct and do not use the same receptor. Here, we investigated whether HCoV-EMC and SARS-CoV induce similar or distinct host responses after infection of a human lung epithelial cell line. HCoV-EMC was able to replica  ...[more]

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