Transcriptomics

Dataset Information

3

Expression data from pig oviduct in response to X or Y chromosome bearing spermatozoa


ABSTRACT: The objective of the present study is to investigate if females have the ability to recognise X or Y chromosome bearing spermatozoa and present a different response to different spermatozoa. In order to investigate if females have the ability to recognise X or Y chromosome bearing spermatozoa and present a different response to different spermatozoa. Samples of semen were sorted for chromosomal sex by flow cytometric-sorting. Using laparoscopic insemination one oviduct was inseminated with X spermatozoa and the contralateral oviduct was inseminated with Y spermatozoa.This allowed us to obtain samples from the oviduct in contact with X sperm and Y sperm in the the contrlateral oviduct from the same sow. Oviduct pig samples were collected for RNA isolation and hybridization on Affymetrix microarrays. Four biological replicates were performed (n= 4 sows) and a total of 8 arrays were used for microarrays study (4 arrays for oviduct samples in contact with X spermatozoa and 4 arrays for oviduct samples in contact with Y spermatozoa.

ORGANISM(S): Sus scrofa  

SUBMITTER: Ignacio Caballero   Jose L Vazquez  Cristina Cuello  Emilio A Martinez  Juan M Vazquez  Maria A Gil  Inmaculada Parrilla  Paul R Heath  William V Holt  Afsaneh S Maleki-Dizaji  Alireza Fazeli  Carmen Alminana  Jordi Roca 

PROVIDER: E-GEOD-47139 | ArrayExpress | 2014-05-20

SECONDARY ACCESSION(S): GSE47139PRJNA203974

REPOSITORIES: GEO, ArrayExpress

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