Dataset Information


Sequencing and genome-wide mapping of 146 bp mono-nucleosomal DNA from human and bovine sperm

ABSTRACT: The phenomenon of nucleosome retention in mammalian sperm chromatin is still not clarified yet. The goal of our study was to characterize the binding sites of sperm nucleosomes in the human and bovine genomes, and through this, to clarify whether nucleosome retention in sperm underlies rules of great generality and has a biological function. Comparing two mammalian systems we found that nucleosomes remain mainly in centromeres and in non-coding intergenic and intron regions. In contrast, coding DNA, promoter regions and transcription start and end sites, especially in homeobox genes and in the majority of genes with relevance for organ development and morphogenesis, were nucleosome-free. 146 bp mono-nucleosomal DNA was isolated and purified from sperm samples of two fertile bulls (bos taurus), one fertile man and a pool of four fertile donors; each DNA sample was deep sequenced using Illumina GAIIx and genome-wide mapped on respective genome; nucleosome-binding sites were analyzed in a genome-wide comparative manner.

SUBMITTER: B Samans   Birgit Samans  U Schagdarsurengin 

PROVIDER: E-GEOD-47843 | ArrayExpress | 2014-07-08



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Uniformity of nucleosome preservation pattern in Mammalian sperm and its connection to repetitive DNA elements.

Samans Birgit B   Yang Yang Y   Krebs Stefan S   Sarode Gaurav Vilas GV   Blum Helmut H   Reichenbach Myriam M   Wolf Eckhard E   Steger Klaus K   Dansranjavin Temuujin T   Schagdarsurengin Undraga U  

Developmental cell 20140703 1

Nucleosome-to-protamine exchange during mammalian spermiogenesis is essential for compaction and protection of paternal DNA. It is interesting that, depending on the species, 1% to 15% of nucleosomes are retained, but the generalizability and biological function of this retention are unknown. Here, we show concordantly in human and bovine that nucleosomes remained in sperm chromatin predominantly within distal intergenic regions and introns and associated with centromere repeats and retrotranspo  ...[more]

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