Transcriptomics

Dataset Information

4

Response of Lactococcus lactis to isoleucine starvation


ABSTRACT: For the first time in Lactococcus lactis, amino acid starvation response was characterized. The natural imposition of isoleucine starvation, by its consumption during growth, associated to transcript profiling, allowed defining exhaustively this stress stimulon. It consisted of a general induction of nitrogen metabolism (amino acid biosynthesis and transport, proteolytic system and proteases), a strong repression of genes encoding major physiological activities (translation, transcription, carbon metabolism, purine and pyrimidine biosynthesis and fatty acid metabolism) and the induction of unexpected cross responses to acid, osmotic and oxidative stresses. Keywords: stress response, time course Isoleucine starvation was imposed by the consumption of this amino acid during the growth of Lactococcus lactis IL1403 on ILV0.1 medium (CDM with ten-fold reduced concentrations of isoleucine, leucine and valine) and under controlled conditions (30 °C, pH 6.6, nitrogen atmosphere). Cell samples were harvested in exponential phase and after 30 min, 1.7 h and 3.5 h of isoleucine starvation. Total RNA was extracted from these samples and radiolabelled cDNA were prepared and hybridized on nylon arrays. 2053 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. The 4 time-points were analyzed simultaneously and 3 independent repetitions were performed.

ORGANISM(S): Lactococcus lactis  

SUBMITTER: Emma Redon   Muriel Cocaign-Bousquet  Pascal Loubiere 

PROVIDER: E-GEOD-4872 | ArrayExpress | 2010-06-25

SECONDARY ACCESSION(S): GSE4872PRJNA95935

REPOSITORIES: GEO, ArrayExpress

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Publications

Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responses.

Dressaire Clémentine C   Redon Emma E   Milhem Helene H   Besse Philippe P   Loubière Pascal P   Cocaign-Bousquet Muriel M  

BMC genomics 20080721


BACKGROUND: The development of transcriptomic tools has allowed exhaustive description of stress responses. These responses always superimpose a general response associated to growth rate decrease and a specific one corresponding to the stress. The exclusive growth rate response can be achieved through chemostat cultivation, enabling all parameters to remain constant except the growth rate. RESULTS: We analysed metabolic and transcriptomic responses of Lactococcus lactis in continuous cultures a  ...[more]

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