Dataset Information

Hot spots of DNA double-strand breaks and genomic contacts of human rDNA units are involved in epigenetic regulation [rDNA units]

ABSTRACT: 4C procedure was used for analysis of genomic contacts of rDNA units in HEK 293T cells. The primers for 4C were selected inside IGS. Our data indicate that mostly rDNA units exhibit close proximity with pericentromeric regions in different chromosomes. We also detected the contacts within a rDNA unit and between rDNA units. Examination of rDNA genome-wide contacts in HEK 293T cells using 4C approach.

OTHER RELATED OMICS DATASETS IN: PRJNA150821PRJNA213579PRJNA213263

ORGANISM(S): Homo sapiens

SUBMITTER: Yuri V Kravatsky Olga V Kretova Nickolai Tchurikov Nickolai A Tchurikov

PROVIDER: E-GEOD-49193 | ArrayExpress | 2014-10-04

SECONDARY ACCESSION(S): GSE49193SRP028241PRJNA213263

REPOSITORIES: GEO, ArrayExpress, ENA

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			Action	DRS
	E-GEOD-49193.idf.txt	Idf
	E-GEOD-49193.processed.1.zip	Processed
	E-GEOD-49193.sdrf.txt	Txt

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Hot spots of DNA double-strand breaks and genomic contacts of human rDNA units are involved in epigenetic regulation.

Tchurikov Nickolai A NA Fedoseeva Daria M DM Sosin Dmitri V DV Snezhkina Anastasia V AV Melnikova Nataliya V NV Kudryavtseva Anna V AV Kravatsky Yuri V YV Kretova Olga V OV

Journal of molecular cell biology 20141003 4

DNA double-strand breaks (DSBs) are involved in many cellular mechanisms, including replication, transcription, and genome rearrangements. The recent observation that hot spots of DSBs in human chromosomes delimit DNA domains that possess coordinately expressed genes suggests a strong relationship between the organization of transcription patterns and hot spots of DSBs. In this study, we performed mapping of hot spots of DSBs in a human 43-kb ribosomal DNA (rDNA) repeated unit. We observed that ...[more]

PMID: 25280477

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