Transcriptomics

Dataset Information

297

RNA seq in two lines of E14 mouse embryonic stem cells


ABSTRACT: This experiment was designed to obtain the polyA+ transcriptome in E14 ESCs PolyA+ RNA was extracted and purified from two separate clones of E14, which were treated as biological replicate

ORGANISM(S): Mus musculus  

SUBMITTER: Syuzo Kaneko   Roberto Bonasio  Danny Reinberg 

PROVIDER: E-GEOD-49434 | ArrayExpress | 2013-10-19

SECONDARY ACCESSION(S): GSE49434SRP028385PRJNA213976

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

PRC2 binds active promoters and contacts nascent RNAs in embryonic stem cells.

Kaneko Syuzo S   Son Jinsook J   Shen Steven S SS   Reinberg Danny D   Bonasio Roberto R  

Nature structural & molecular biology 20131020 11


EZH2 is the catalytic subunit of PRC2, a central epigenetic repressor essential for development processes in vivo and for the differentiation of embryonic stem cells (ESCs) in vitro. The biochemical function of PRC2 in depositing repressive H3K27me3 marks is well understood, but how it is regulated and directed to specific genes before and during differentiation remains unknown. Here, we report that PRC2 binds at low levels to a majority of promoters in mouse ESCs, including many that are active  ...[more]

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