Transcriptomics

Dataset Information

0

Eye-Specific Gene Expression following Embryonic Ethanol Exposure in Zebrafish: Roles for Heat Shock Factor 1


ABSTRACT: Ethanol is a well-known teratogen. While this teratogenic potential is well-characterized clinically, the mechanisms through which ethanol exposure results in developmental defects remain unclear. Here we use the zebrafish model to elucidate eye-specific mechanisms that may underlie ethanol-mediated microphthalmia (reduced eye size), using time-series microarray analysis of gene expression of eye tissues of embryos exposed to 1.5% ethanol vs. untreated embryos. We identified 62 genes differentially expressed in ethanol-treated as compared to control zebrafish eyes from all sampling times over the period of retinal neurogenesis (24-48 hours post-fertilization). Application of the EDGE (extraction of differential gene expression) algorithm identified over 3000 genes differentially expressed over developmental time in ethanol-treated embryo eyes as compared to untreated embryo eyes. These lists included several genes indicating a mis-regulated cellular stress response (heat shock response) due to ethanol exposure. Combined treatment with sub-threshold levels of ethanol and a morpholino (MO) targeting heat shock factor 1 (hsf-1) mRNA resulted in a microphthalmic phenotype, suggesting convergent molecular pathways. Manipulation of the heat shock response by thermal preconditioning partially prevented ethanol-mediated microphthalmia while maintaining Hsf-1 expression. Together these data are consistent with roles for reduced Hsf-1 in mediating microphthalmic effects of embryonic ethanol exposure in zebrafish. time series, 9 samples, no replicates

ORGANISM(S): Danio rerio  

SUBMITTER: Bhavanim Kashyap   Deborah Stenkamp  Deborah Lynn Stenkamp 

PROVIDER: E-GEOD-51427 | ArrayExpress | 2014-01-01

SECONDARY ACCESSION(S): GSE51427PRJNA223154

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2007-02-26 | BIOMD0000000091 | BioModels Database
| PRJNA223154 | ENA
| GSE81520 | GEO
| GSE81522 | GEO
| GSE81521 | GEO
2010-03-01 | GSE20424 | GEO
2010-05-06 | E-GEOD-20424 | ArrayExpress
2010-09-02 | GSE19025 | GEO
2010-09-02 | E-GEOD-19025 | ArrayExpress
| GSE77607 | GEO