Genomics

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Genome-wide map of RNA polymerase II, NELF, and DSIF in HeLa cells


ABSTRACT: Most metazoan promoters have RNA polymerase II (Pol II) paused slightly downstream of the transcription start site by NELF and DSIF. This pausing keeps these promoters available for rapid induction by P-TEFb, whose activity causes NELF to dissociate and Pol II and DSIF to elongate on the gene. ChIP-Seq data was generated for Pol II, NELF, and DSIF in HeLa cells and used to look at pausing downstream of unannotated promoters. 3x ChIP-Seq (Pol II, NELF, DSIF) in HeLa cells

ORGANISM(S): Homo sapiens  

SUBMITTER: Kyle Nilson   David H Price  Kyle A Nilson 

PROVIDER: E-GEOD-53008 | ArrayExpress | 2014-02-01

SECONDARY ACCESSION(S): GSE53008SRP033525PRJNA230673

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Release of positive transcription elongation factor b (P-TEFb) from 7SK small nuclear ribonucleoprotein (snRNP) activates hexamethylene bisacetamide-inducible protein (HEXIM1) transcription.

Liu Pingyang P   Xiang Yanhui Y   Fujinaga Koh K   Bartholomeeusen Koen K   Nilson Kyle A KA   Price David H DH   Peterlin B Matija BM  

The Journal of biological chemistry 20140210 14


By phosphorylating negative elongation factors and the C-terminal domain of RNA polymerase II (RNAPII), positive transcription elongation factor b (P-TEFb), which is composed of CycT1 or CycT2 and CDK9, activates eukaryotic transcription elongation. In growing cells, it is found in active and inactive forms. In the former, free P-TEFb is a potent transcriptional coactivator. In the latter, it is inhibited by HEXIM1 or HEXIM2 in the 7SK small nuclear ribonucleoprotein (snRNP), which contains, add  ...[more]

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