Genomics

Dataset Information

239

MOF-associated complexes have overlapping and unique roles in regulating pluripotency in embryonic stem cells and during differentiation [ChIP-seq]


ABSTRACT: The histone acetyltransferase (HAT) Mof is essential for mouse embryonic stem cells (mESC) pluripotency and early development. Mof is the enzymatic subunit of two different HAT complexes, MSL (Male-Specific Lethal) and NSL (Non-specific lethal). The individual contribution of MSL and NSL complexes to transcription regulation in mESCs is not well understood. Our genome-wide analysis of MSL and NSL localization show that i) MSL and NSL bind to specific and common sets of expressed genes, ii) NSL binds at promoters, iii) while MSL binds in gene bodies. Knockdown of Msl1 leads to a global loss of histone H4K16ac indicating that MSL is the main HAT acetylating H4K16 in mESCs. MSL was enriched at many mESC-specific genes, but also at bivalent domains. Thus, NSL and MSL HAT complexes differentially regulate specific sets of expressed genes in mESCs. Furthermore, MSL is essential for the regulation of key mESC-specific and bivalent developmental genes. Genome-wide comparions of Msl1 and Nsl1 binding in mESCs

ORGANISM(S): Mus musculus  

SUBMITTER: Laszlo Tora   Sarina Ravens  Doulaye Dembele  Tao Ye  Marjorie Fournier  Matthieu Stierle  Virginie Chavant 

PROVIDER: E-GEOD-53797 | ArrayExpress | 2014-06-04

SECONDARY ACCESSION(S): SRP034893GSE53797PRJNA233307

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Mof-associated complexes have overlapping and unique roles in regulating pluripotency in embryonic stem cells and during differentiation.

Ravens Sarina S   Fournier Marjorie M   Ye Tao T   Stierle Matthieu M   Dembele Doulaye D   Chavant Virginie V   Tora Làszlò L  

eLife 20140604


The histone acetyltransferase (HAT) Mof is essential for mouse embryonic stem cell (mESC) pluripotency and early development. Mof is the enzymatic subunit of two different HAT complexes, MSL and NSL. The individual contribution of MSL and NSL to transcription regulation in mESCs is not well understood. Our genome-wide analysis show that i) MSL and NSL bind to specific and common sets of expressed genes, ii) NSL binds exclusively at promoters, iii) while MSL binds in gene bodies. Nsl1 regulates p  ...[more]

Similar Datasets

2014-06-04 | E-GEOD-56646 | ArrayExpress
2014-06-03 | E-GEOD-57698 | ArrayExpress
2013-02-15 | E-GEOD-44252 | ArrayExpress
2011-10-28 | E-GEOD-30991 | ArrayExpress
2014-06-03 | E-GEOD-51746 | ArrayExpress
2015-11-06 | E-GEOD-69671 | ArrayExpress
2011-10-28 | E-GEOD-30989 | ArrayExpress
2008-06-09 | E-MEXP-1505 | ArrayExpress
2011-10-28 | E-GEOD-30990 | ArrayExpress
2008-06-12 | E-MEXP-1508 | ArrayExpress