Dataset Information


Gene expression in primary human macrophages and foam cells

ABSTRACT: The aim of the experiment was to determine the effects of 48 hours of treatment with oxidized low density lipoprotein (oxLDL) on gene expression in primary human monocyte-derived macrophages. 6 independent biological replicates were processed. From each replicate half of the cells were treated with oxLDL and the other half were treated with the control buffer. For foam cell formation (after 7 days) macrophages were treated with oxLDL (50mcg/ml, enodtoxin free, Copper oxidized). Control macrophages were treated with a matching buffer without oxLDL. Treatment duration - 48 hours.

ORGANISM(S): Homo sapiens  

SUBMITTER: Michael E Reschen   Christopher O'Callaghan 

PROVIDER: E-GEOD-54666 | ArrayExpress | 2015-04-08



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Lipid-induced epigenomic changes in human macrophages identify a coronary artery disease-associated variant that regulates PPAP2B Expression through Altered C/EBP-beta binding.

Reschen Michael E ME   Gaulton Kyle J KJ   Lin Da D   Soilleux Elizabeth J EJ   Morris Andrew J AJ   Smyth Susan S SS   O'Callaghan Christopher A CA  

PLoS genetics 20150402 4

Genome-wide association studies (GWAS) have identified over 40 loci that affect risk of coronary artery disease (CAD) and the causal mechanisms at the majority of loci are unknown. Recent studies have suggested that many causal GWAS variants influence disease through altered transcriptional regulation in disease-relevant cell types. We explored changes in transcriptional regulation during a key pathophysiological event in CAD, the environmental lipid-induced transformation of macrophages to lipi  ...[more]

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