Transcriptomics

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RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides


ABSTRACT: A comparative RNA-seq approach between R. sphaeroides 2.4.1 wild type and an RNase J deletion strain 2.4.1∆rnj revealed the accumulation of different mRNA fragments in the mutant. The structural characteristics of these RNA fragments suggest that RNase J is responsible for the decay of degradation intermediates that cannot serve as substrates for the 3´-to-5´ exoribonucleases. Total RNA from exponentially grown cultures (OD660 0,4) of 2.4.1 wild type and 2.4.1∆rnj has been isolated in triplicates using the hot phenol method and prepared for sequencing on a Illumina Genome Analyzer IIx machine.

ORGANISM(S): Rhodobacter sphaeroides 2.4.1  

SUBMITTER: Gabriele Klug  Tom Rische   Konrad U Förstner   Konrad U. Förstner    

PROVIDER: E-GEOD-54750 | ArrayExpress | 2014-02-06

SECONDARY ACCESSION(S): GSE54750SRP036835PRJNA237547

REPOSITORIES: GEO, ArrayExpress, ENA

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