Transcriptomics

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Transcript data affected by constitutive expression of VvMYBPAR in Arabidopsis


ABSTRACT: Constitutive expression of VvMYBPAR in Arabidopsis was found to accumulate proanthocyanidins when the plants were grown on sucrose-supplemented media to induce anthocyanins. To identify the putative targets of VvMYBPAR, the transcriptome analysis of the transgenic lines which highly express VvMYBPAR was carried out using NimbleGen microarray. Three transgenic lines in which VvMYBPAR were constitutively expressed under the control of 35S promoter vs. empty vector transformants were compared.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Kazuya Koyama   Nobukazu Tanaka 

PROVIDER: E-GEOD-55318 | ArrayExpress | 2014-06-03

SECONDARY ACCESSION(S): GSE55318PRJNA239313

REPOSITORIES: GEO, ArrayExpress

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Publications

Functional characterization of a new grapevine MYB transcription factor and regulation of proanthocyanidin biosynthesis in grapes.

Koyama Kazuya K   Numata Mineyo M   Nakajima Ikuko I   Goto-Yamamoto Nami N   Matsumura Hideo H   Tanaka Nobukazu N  

Journal of experimental botany 20140523 15


A new regulator of proanthocyanidin (PA) biosynthesis in grapes was found by screening genes coordinately expressed with PA accumulation under different light conditions using a substantially improved method of serial analysis of gene expression (SuperSAGE). This R2R3-MYB transcription factor, VvMYBPAR, shows high protein sequence similarity with PA biosynthesis-regulating plant MYBs, such as VvMYBPA2 and TRANSPARENT TESTA2. Its transcript levels were relatively high in the skins of young berrie  ...[more]

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